The sustainability of native forests in Sub-Saharan Africa depends on the diversification of sources to generate bioenergy, and Eucalyptus spp. wood has been highlighted. However, the determination of energy quality parameters has been a challenge to enable plantation wood to generate energy. The research assessed the ash content of radial and longitudinal samples of Eucalyptus grandis (Hill) clone with different ages and growth sites. Samples were collected in three pre-established plots in the center of Mozambique. Five trees were cut down in each plot and six discs were removed from each tree. Grinded samples with <0.5 mm particle size were generated from the heartwood and sapwood of each disk to determine the ash content. Wood from 7-year-olds had a higher ash content compared to 9-year-olds. The two sample plots differed from each other in terms of wood ash content. Heartwood samples had smaller ash content than sapwood samples. In general, the ash content of the intermediate positions was lower than those from the base and top of the stem, for both radial sections. No conclusive differences were found between samples from the base and the top of the trees, indicating that the material from the top of the trees can also be used as wood fuel. Ash content can be a considerable parameter to assess the quality of the wood of Eucalyptus spp. as a fuel.