%0 Research article
%T Ophiostomatoid fungi transported by Ips sexdentatus (Coleoptera; Scolytidae) in Pinus pinaster in NW Spain
%A Bueno, Alberto
%A Diez, Julio J.
%A Fernández, Mercedes M.
%D 2010
%J Silva Fennica
%V 44
%N 3
%R doi:10.14214/sf.137
%U https://silvafennica.fi/article/137
%X Ips sexdentatus (Coleoptera; Scolytidae) is one of the main vectors of  ophiostomatoid blue stain fungi that can cause mortality of healthy  conifers. For this reason, our objective was to identify the fungal  species carried by this bark beetle in Maritime pine (Pinus pinaster) in  north-western Spain. We collected insects from naturally infected pines  placed them on malt extract agar (MEA) and left to walk freely on  culture plates. Plant tissues (phloem and xylem) from adult pines were  cultivated in moist chambers and also on MEA. At the same time, we  inoculated pine logs with living insects in the laboratory. Four  ophiostomatoid fungi appeared: Ophiostoma ips, Ophiostoma  brunneo-ciliatum, Ceratocystiopsis minuta and Ophiostoma sp., as well as  Graphium and Sporothrix imperfect stages. Moreover there were seven  saprophytic species: Penicillium sp., Trichoderma sp., Verticillium sp.,  Mucor sp., Aspergillus niger, Gliocladium viride and Scopulariopsis  brevicaulis, and the pathogenic Ophiostoma ips. The fructification  percentage of the ophiostomatoid species was low, however; its imperfect  stage Sporothrix/Hyalorhinocladiella produced high quantity of  conidiophores.