Current issue: 53(4)

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Silva Fennica 1926-1997
1990-1997
1980-1989
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1960-1969
Acta Forestalia Fennica
1953-1968
1933-1952
1913-1932

Articles by Márcio Akira Ito

Category: Research article

article id 1691, category Research article
Enéas Ricardo Konzen, Raquel Peron, Márcio Akira Ito, Gilvano Ebling Brondani, Siu Mui Tsai. (2017). Molecular identification of bamboo genera and species based on RAPD-RFLP markers. Silva Fennica vol. 51 no. 4 article id 1691. https://doi.org/10.14214/sf.1691
Highlights: We investigated the potential of RAPD-RFLP (Random Amplified Polymorphic DNA, Restriction Fragment Length Polymorphism) in detecting genetic relationships among bamboo genera and species; RFLP profiles resolved in acrylamide gels revealed high number of markers, which accurately differentiated species and genera, based on cophenetic correlation coefficients; We recommend RAPD-RFLP for analyses of genetic diversity and divergence among bamboo genera, species and varieties.

Bamboo species have a very significant ecological and economic impact. Determining morphological and genetic differences among bamboo genera and species are crucial to explore desirable traits for breeding purposes. Several advances have been made in the taxonomy of bamboos by using molecular fingerprinting tools and next generation sequencing technologies. Nevertheless, classical molecular markers such as RAPD (Random Amplified Polymorphic DNA), AFLP (Amplified Fragment Length Polymorphism) and ISSR (Inter Simple Sequence Repeats) also provide an accurate discrimination among genera and species. Moreover, the RAPD-RFLP (Random Amplified Polymorphic DNA, Restriction Fragment Length Polymorphism) method, in which amplification products from RAPD are digested with restriction enzymes, is a reliable, fast and cost-effective method for fingerprinting. RAPD-RFLP has been scarcely used in the literature and no report regarding bamboo taxonomy is available with this method. Here we explored the molecular (RAPD, RAPD-RFLP) variation among genera (Bambusa, Dendrocalamus, Guadua and Phyllostachys) and species of bamboo cultivated in Brazil. Both molecular markers allowed clear distinction among the genera studied. Moreover, high cophenetic correlation values in UPGMA clusters indicated their potential for discriminating bamboo species. The digestion of RAPD products (RFLP) resulted in high number of polymorphic bands and produced very characteristic profiles for each genus with three enzyme combinations (HindIII/HaeIII, HinfI/RsaI, and single digestion with MspI). We recommend RAPD-RFLP as a reproducible and informative method for screening differences among genera, species and varieties of bamboos. Providing a cost-effective and accurate method for species identification and characterization is straightforward for bamboo conservation, management and breeding.

  • Konzen, Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of Sao Paulo (USP), Centenário Av., 303, Piracicaba, SP, P.O. Box 96, Brazil; Forest Sciences, Federal University of Lavras (UFLA), Lavras, MG, P.O. Box 3037, Brazil ORCID ID:E-mail: erkonzen@gmail.com (email)
  • Peron, Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of Sao Paulo (USP), Centenário Av., 303, Piracicaba, SP, P.O. Box 96, Brazil ORCID ID:E-mail: rperon@purdue.edu
  • Ito, Embrapa Western Agriculture, Brazilian Agricultural Research Corporation (EMBRAPA), BR 163 Rd., km 253, Dourados, MS, P.O. Box 449, Brazil ORCID ID:E-mail: marcio.ito@embrapa.br
  • Brondani, Forest Sciences, Federal University of Lavras (UFLA), Lavras, MG, P.O. Box 3037, Brazil ORCID ID:E-mail: gebrondani@gmail.com
  • Tsai, Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of Sao Paulo (USP), Centenário Av., 303, Piracicaba, SP, P.O. Box 96, Brazil ORCID ID:E-mail: tsai@cena.usp.br

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