Under changing environmental conditions, biomass development on the tree and the stand level may differ from today, regardless if the induced change is due to a shift in the general climate properties or to forest management. Under these conditions, tree biomass can not be derived from tables based on former investigations but has to be defined from particular biomass investigations, which generally calculate tree and stand biomass from sample branches using allometric relationships. Therefore, sample measurements on harvested trees are needed. In this paper, foliage and branch biomass estimation for 6 Norway spruces (Picea abies) and 6 beeches (Fagus sylvatica) harvested in a 56-year-old mixed stand in southern Germany is presented. Different allometric models are investigated to derive branch biomass from branch dimension for both species. The equations that are based on branch length, foliated branch fraction, and branch diameter are used for tree and stand level estimates. However, the variation within the 6 trees of each species was too large for a reliable calculation of stand biomass, especially in case of beech branch wood. Furthermore, the necessity of allometric relations and their applicability in individual-tree models is discussed, and the importance of suitable branch- and tree selection is underlined.