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Articles containing the keyword 'RAPD'.

Category: Research article

article id 1283, category Research article
Ivana Bjedov, Dragica Obratov–Petković, Danijela Mišić, Branislav Šiler, Jelena M Aleksic. (2015). Genetic patterns in range-edge populations of Vaccinium species from the central Balkans: implications on conservation prospects and sustainable usage. Silva Fennica vol. 49 no. 4 article id 1283. https://doi.org/10.14214/sf.1283
Highlights: We studied fragmentary distributed range-edge populations of Vaccinium myrtillus, Vaccinium uliginosum and Vaccinium vitis-idaea from the Balkans using RAPDs; Low genetic diversities and high genetic differentiation were found in all species; The prevalence of clonal individuals was not observed; Past interspecific hybridization among V. vitis-idaea and the other two species was detected; Guidelines for conservation and sustainable usage were provided.

Vaccinium myrtillus L., Vaccinium uliginosum L. and Vaccinium vitis-idaea L. are perennial, cold-adapted clonal shrubs distributed throughout Europe, northern Asia and North America. Due to their usage in food (berries) and pharmaceutical industry (berries and leaves), their natural populations are exposed to anthropogenic and other impacts that affect their genetic make-up. We analyzed 14 fragmentary distributed and small-sized peripheral populations of these species from the Balkans, which represents the southeastern-European marginal area of their wide European distributions, using RAPD molecular markers. The contemporary genetic patterns in all three species within the Balkans were generally similar, and in comparison to previous reports on populations of these species found in northward Europe, where they have a more continuous distribution, the levels of genetic diversity were more or less halved, genetic differentiation was several times higher, gene flow exceptionally low, and the expected prevalence of clonal individuals was lacking. The population dynamics of all three species within the Balkans was complex and distinct, and was characterized by a past admixture of individuals from discrete populations of the same species and interspecific hybridisation not only between V. myrtillus and V. vitis-idaea but also between V. uliginosum and V. vitis-idaea, the latter not being reported to date. Conservation measures suitable for preservation of presumably genetically distinct portions of the Balkans’ gene pools of studied species have been suggested, while the utility of interspecific hybrids in breeding programs and/ or in food/pharmaceutical industry is yet to be assessed. 

  • Bjedov, University of Belgrade, Faculty of Forestry, Kneza Višeslava 1, 11000 Belgrade, Serbia ORCID ID:E-mail: ivana.bjedov@sfb.bg.ac.rs
  • Obratov–Petković, University of Belgrade, Faculty of Forestry, Kneza Višeslava 1, 11000 Belgrade, Serbia ORCID ID:E-mail: dragica.obratov-petkovic@sfb.bg.ac.rs
  • Mišić, University of Belgrade, Institute for Biological Research “Siniša Stanković”, Boulevard Despota Stefana 142, 11000 Belgrade, Serbia ORCID ID:E-mail: dmisic@ibiss.bg.ac.rs
  • Šiler, University of Belgrade, Institute for Biological Research “Siniša Stanković”, Boulevard Despota Stefana 142, 11000 Belgrade, Serbia ORCID ID:E-mail: branislav.siler@ibiss.bg.ac.rs
  • Aleksic, University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia ORCID ID:E-mail: aleksic_jelena@yahoo.com.au (email)
article id 229, category Research article
Carmen Martín, Teresa Parra, Margarita Clemente-Muñoz, Esteban Hernández-Bermejo. (2008). Genetic diversity and structure of the endangered Betula pendula subsp. fontqueri populations in the south of Spain. Silva Fennica vol. 42 no. 4 article id 229. https://doi.org/10.14214/sf.229
Betula pendula subsp. fontqueri, present in the south of Spain, has been considered in danger of extinction and, for this reason, some regional governments in Spain have included their populations in conservation programmes. In order to establish the genetic structure of the Betula pendula subsp. fontqueri populations, a random amplified polymorphic DNA (RAPD) analysis was carried out. Two B. pubescens populations were included in the study as taxonomic controls. B. pendula subsp. fontqueri populations were clearly differentiated through UPGMA, and showed significant pairwise genetic distance (ΦST) values between all pairs of populations obtained by AMOVA. Genetic diversity found between populations was not correlated to geographical distances. The significant differences among populations must be due to progressive isolation of Betula populations along their paleogeographical history, and more recently to the drastic fragmentation and reduction of some of these populations. The results obtained in this work show clear genetic differences which could be considered in the management of conservation strategies for Betula pendula subsp. fontqueri in its Iberian meridional distribution.
  • Martín, Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos de Madrid, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain ORCID ID:E-mail: mariacarmen.martin@upm.es (email)
  • Parra, Departamento de Ciencias y Recursos Agrícolas y Forestales, Universidad de Córdoba, Avda. Linneo s/n, 14004-Córdoba, Spain ORCID ID:E-mail:
  • Clemente-Muñoz, Departamento de Ciencias y Recursos Agrícolas y Forestales, Universidad de Córdoba, Avda. Linneo s/n, 14004-Córdoba, Spain ORCID ID:E-mail:
  • Hernández-Bermejo, Departamento de Ciencias y Recursos Agrícolas y Forestales, Universidad de Córdoba, Avda. Linneo s/n, 14004-Córdoba, Spain ORCID ID:E-mail:
article id 291, category Research article
Yildiray Lise, Zeki Kaya, Fikret Isik, Rumi Sabuncu, Irfan Kandemir, Sertaç Önde. (2007). The impact of over-exploitation on the genetic structure of Turkish red pine (Pinus brutia Ten.) populations determined by RAPD markers. Silva Fennica vol. 41 no. 2 article id 291. https://doi.org/10.14214/sf.291
To determine the possible impact of over-exploitation on the genetic structure of Turkish red pine (Pinus brutia Ten.) populations, three natural and three over-exploited (human degraded) populations of the species in the Mediterranean region of Turkey were investigated with Randomly Amplified Polymorphic DNA (RAPD). With the 80 RAPD primers tested, 12 of them yielded 137 polymorphic RAPD fragments. Four of the studied populations maintained unique fragments. The mean proportion of polymorphic fragments for all populations ranged from 89.8 to 98.9% and there were no significant differences between natural (94.8%) vs. over-exploited populations (92.7%). The estimated heterozygosity values suggested that Turkish red pine maintains high levels of genetic diversity (range 0.24–0.28) though studied populations and grouped ones as natural (He = 0.28) vs. over-exploited (0.27) did not differ significantly. The mean FST value indicated that the large portion of the total genetic diversity was within populations (93%), but this value was lower in the natural populations (92%) than in the over-exploited ones (94%). In over-exploited populations, excess of homozygosity was observed (about 6% higher) as compared to natural populations, indicating impacts of inbreeding in P. brutia.
  • Lise, Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey ORCID ID:E-mail:
  • Kaya, Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey ORCID ID:E-mail: kayaz@metu.edu.tr (email)
  • Isik, North Carolina State University, Raleigh, USA ORCID ID:E-mail:
  • Sabuncu, Southwest Anatolia Forest Research Institute, Antalya, Turkey ORCID ID:E-mail:
  • Kandemir, Department of Biology, Zonguldak Karaelmas University, 67100, Zonguldak, Turkey ORCID ID:E-mail:
  • Önde, Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey ORCID ID:E-mail:

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