Current issue: 54(2)
The availability of appropriate propagation techniques is a major constraint to the domestication of the forest trees widely used by rural communities; such as Garcinia kola (Heckel). This study tested the ability of root and stem cuttings to regenerate vegetatively when treated with indole-3-butyric acid (IBA), and set in non-mist poly-propagator within a shaded nursery. It found that G. kola is amenable to propagation by softwood stem cuttings. Attention was given to the effect of cutting age (softwood, semi hardwood) and IBA application with regard to the sprouting and rooting efficiency. Bud and leaf emergence time were also investigated, as was the coppicing ability of the stump. Results revealed that roots and branches from mature forest trees did not sprout under any culture conditions. The highest percentages of rooting (70–85%) were obtained with softwood cuttings set in non-mist poly-propagator, regardless of hormonal treatment. The mean numbers of buds (2.9 ± 0.4) and roots (2.6 ± 0.1) produced by softwood cuttings was significantly greater than those obtained in the Control. The best average emergence time of buds (25.1 days ± 9.3) and leaves (36.8days ± 8.4) was obtained with cuttings treated with IBA and set in non-mist poly-propagator against, 54.4 ± 12.5 and 72.6 ± 3.4 days respectively for the Control. In general, non-mist poly-propagator gave the best propagation results. When coppiced, the shoots emerging from stumps with one node were the most vigorous.
Forest tree breeding involves manipulation of genetic composition of populations and individuals, and biotechnology focuses on selected individuals. The new techniques cannot replace the conventional breeding techniques but both need effective cooperation of each other. Thus, the distinction between conventional breeding and biotechnology is artificial. The biotechnology methods are new and fast developing and the future with field and progeny testing will show which techniques will be permanently adopted into tree breeding. For instance, the earlier hope of the use of somaclonal variation as a new source of variability and a powerful tool for the breeder seem today quite the opposite. Somaclonal variation constituting a major problem in present-day micropropagation is due to the unpredictable variation. Based on knowledge of today, especially micropropagation via somatic embryos, transgenic trees and the identification of major genes seem to be good candidates to be permanently adopted into tree breeding.
Needle fascicles of Scots pine (Pinus sylvestris L.) were rooted in a standard Jacobsen’s germination apparatus. The apparatus was found to be suitable for rooting at least on laboratory scale. The best rooting substrate was living Sphangnum, which remained sufficiently moist in the germination containers throughout the experiments. In a comparison of various growth substrate treatments, the best result was obtained with a combination of IAA (100 mg/l) and thiamine (5 mg/l). The rooting percentage using these growth substances with Sphagnum as the rooting medium was in the first experiment 30 and in the second 48.
The PDF includes a summary in Finnish.