Current issue: 54(2)

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Silva Fennica 1926-1997
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Acta Forestalia Fennica
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Articles containing the keyword 'viability'.

Category: Research article

article id 10132, category Research article
Yan Liu, Yuan Zhang, Qing Zhou, Jian Wu, Pingdong Zhang. (2019). Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa. Silva Fennica vol. 53 no. 2 article id 10132. https://doi.org/10.14214/sf.10132
Highlights: The number of colchicine injections and the meiotic stage at which they were administered both had a significant effect on the occurrence rate of induced 2n pollen in Populus tomentosa; Treating male buds with 5000 ppm colchicine solution during meiosis led to a significant decrease in pollen production; Colchicine injection could induce P. tomentosa to produce 2n pollen and did not lead to dysfunction of induced diploid pollen.

Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination in vitro and crossing induced pollen with normal gametes to produce triploid in Populus tomentosa Carrière. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed (p < 0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce P. tomentosa to produce 2n pollen and will not lead to dysfunction of induced diploid pollen.

  • Liu, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China ORCID ID:E-mail: 342767649@qq.com
  • Zhang, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China ORCID ID:E-mail: 409192881@qq.com
  • Zhou, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China ORCID ID:E-mail: 876034493@qq.com
  • Wu, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China ORCID ID:E-mail: 1269485709@qq.com
  • Zhang, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China ORCID ID:E-mail: zhangpd@bjfu.edu.cn (email)

Category: Research note

article id 646, category Research note
K. S. Krishna Chaitanya, S. Keshavkant, S. C. Naithani. (2000). Changes in total protein and protease activity in dehydrating recalcitrant sal (Shorea robusta) seeds. Silva Fennica vol. 34 no. 1 article id 646. https://doi.org/10.14214/sf.646
A rapid loss of viability was recorded in sal seeds when dehydrated below 36.7% moisture content, at ambient conditions. Seed becomes non-viable at 6 days after harvest (dah). Gradual decline in total protein content due to corresponding increase in protease activity preceded loss of viability. Almost 43.7% and 52.6% loss in total protein content was observed on 3 dah in embryonic axis and cotyledon respectively of seeds showing 100% viability. No protease activity was detected in the embryonic axis and cotyledon of freshly harvested viable seeds (0 dah). The protease activity was detected after 12 hrs of storage and increased sharply with peak levels on 6 dah (0.71 ± 0.04 / min/mg protein) and 4 dah (0.16 ± 0.01 / min/mg protein) in embryonic axis and cotyledon respectively. Later on the enzyme activity declined sharply in both the tissues. Enhanced protease activity in embryonic axes and cotyledons has been discussed with corresponding decline in total protein during desiccation induced loss of viability in sal seeds.
  • Chaitanya, Pt. Ravishankar Shukla University, Seed Biology Lab, School of Life Sciences, Raipur – 492 010 (M.P.), India ORCID ID:E-mail:
  • Keshavkant, Pt. Ravishankar Shukla University, Seed Biology Lab, School of Life Sciences, Raipur – 492 010 (M.P.), India ORCID ID:E-mail: sk@n.in
  • Naithani, Pt. Ravishankar Shukla University, Seed Biology Lab, School of Life Sciences, Raipur – 492 010 (M.P.), India ORCID ID:E-mail: naithani@mantraonline.com (email)

Category: Article

article id 5571, category Article
Katarina Lindgren, Dag Lindgren. (1996). Germinability of Norway spruce and Scots pine pollen exposed to open air. Silva Fennica vol. 30 no. 1 article id 5571. https://doi.org/10.14214/sf.a9216

Germination of Norway spruce (Picea abies (L.) H. Karst.) and Scots pine (Pinus sylvestris L.) pollen decreased during exposure to open air conditions. Usually more than half of the pollen remained germinative after a few days outdoors, but following more than four days outdoors the germination became very low. This study supports the opinion that pollen in the atmosphere remains viable long enough to allow for long-distance gene flow by pollen migration, as an important factor in genetic management of conifers and in evolution, maintaining diversity and potential for adaptation.

  • Lindgren, ORCID ID:E-mail:
  • Lindgren, ORCID ID:E-mail:
article id 4810, category Article
Pentti Solin. (1970). Kuusen käpyjen säilyttäminen jäähdyttämössä ja sen vaikutus niistä saatavan siemenen itävyyteen. Silva Fennica vol. 4 no. 1 article id 4810. https://doi.org/10.14214/sf.a14598
English title: Cold storage of Norway spruce cones and its effect on seed viability.

The aim of the study was to establish how the cold storage of cones of Norway spruce (Picea abies (L.) H. Karst.) affects the viability of the seeds and the percentage ratio in 7 days. A parallel study was made of the longevity of seed in barn-stored cones subject to weather fluctuations and the longevity of seed extracted immediately and stored in the conventional way in an air-tight container. The cones were collected near Kuopio in Central Finland and near Tampere.

The viability and germination rate of the control sample was constant throughout the storage period. This storage method proved the best. The viability of seeds kept in cones declined in cold storage after 3 ½ months. The cones collected in Tampere were damaged by Laspeyresia strobilella, which affected the viability of the seeds.

The viability of seeds stored in cones in a barn had not weakened by the end of May, however, they deteriorated during the summer, as did the seeds stored in cones in the cold storage. Viability of the seeds was still 94% in October. The germination rate was constant in each lot up to the end of May, after which it decreased to 81.7–86.1% in October.

The results show that healthy spruce cones can be stored in paper sacks in a single layer in cold storage and in an ordinary barn for several months without it affecting the viability of the seeds.

The PDF includes a summary in English.

  • Solin, ORCID ID:E-mail:

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