Table 2. Positions of variable sites in 991 bp long aligned mtDNA nad7 intron1 matrix comprised of six Pinus nigra haplotypes and three Pinus sylvestris haplotypes used as an outgroup. | |||||||||||||||||
Position | |||||||||||||||||
317 | 318 | 328 | 334 | 337 | 342–373 | 392–395 | 396–427 | 428 | Δ 435 | 445–508 | Δ 472 | Δ 520 | 565–614 | 775–805 | 889 | 931–936 | |
Haplotype | A | 1 bp insertion (T) | T | A | T | 32 bp insertion | 4 bp insertion | 32 bp insertion | 1 bp insertion (C) | C | 64 bp insertion | 1 b indel (C) | C | 50 bp insertion | 31 bp insertion | G | 6 bp insertion |
T | . | - | . | . | . | . | . | . | . | . | . | . | . | . | . | . | - |
G | . | - | G | . | . | . | . | . | . | . | . | . | . | . | . | . | - |
h1 | . | - | . | . | . | . | . | . | . | G | . | - | A | . | . | . | - |
h2 | C | - | . | . | . | . | . | . | . | G | . | - | A | . | . | . | - |
h3 | . | . | G | . | . | . | . | . | . | G | . | - | A | . | . | . | - |
h4 | . | - | G | . | . | . | . | . | . | G | . | - | A | . | . | . | - |
PS1 | C | - | . | T | G | . | . | - | . | . | - | - | . | - | - | T | . |
PS2 | C | - | . | T | G | . | - | - | - | . | - | - | . | - | - | T | . |
PS3 | C | - | . | T | G | - | . | - | . | . | - | - | . | - | - | T | . |
T and G – mtDNA haplotypes found in P. nigra individuals from the central Balkans; h1, h2, h3 and h4 – Iberian (h1 – h3) and north African (h4) haplotypes of P. nigra reported by Jaramillo-Correa et al. (2010); PS1, PS2, and PS3 – P. sylvestris haplotypes available in GenBank (accessions DQ665913, DQ665914, and DQ665915, Naydenov et al. 2007); Δ – The positions of mutations which may be used for distinguishing westward and eastward lineages of P. nigra (nucleotide characters “G” and “A” at positions 435 and 520 are synapomorphic for P. nigra individuals from the Iberian Peninsula/northern Africa; one bp insertion (C) at position 472 is synapomorphic for individuals from the central Balkans) |