Table 1. Geographical location of fourteen sampled populations of Tetraclinis articulata and genetic diversity estimates. P percentage of polymorphic loci, H0 Nei’s heterozygosity (genetic diversity), H0* Nei’s heterozygosity calculated for a standardised population size of 10, Hs mean heterozygosity within populations, Ht total heterozygosity between populations, Gst index of population differentiation.
Population Location Individuals
sampled 		(P) %
polymorph. loci 		H0 H0* Hs Ht Gst
Algeria
1 Zemmora 38°42.80´N, 0°45.36´E 10 48.06 0.159 0.159      
2 El Fedjoudj 36°30.40´N, 7°21.70´E 10 45.37 0.178 0.178      
3 Saida 34°50.45´N, 0°04.63´E 10 39.14 0.160 0.160      
Morocco
4 Khenifra 33°05.69´N, 5°35.03´W 10 37.40 0.153 0.153      
5 El Ksiba 32°34.35´N, 6°02.81´W 21 64.63 0.206 0.201      
6 Oujda 34°30.92´N, 1°54.21´W 20 60.98 0.184 0.174      
7 Hassi Berkane 34°43.60´N, 2°47.60´W 10 45.07 0.154 0.154      
8 Ain el Aouda 33°44.66´N, 6°45.05´W 21 68.29 0.204 0.195      
Malta
9 Wied Mizieb 35°51.89´N, 14°24.17´E 31 73.17 0.183 0.154      
10 Argotti 35°53.48´N, 14°30.11´E 14 53.66 0.161 0.169      
11 Gnien Ingraw 35°57.71´N, 14°21.48´E 20 79.27 0.231 0.205      
Spain
12 Cenizas 37°35.49´N, 0°49.67´W 14 69.51 0.227 0.195      
13 Sabinar 37°37.22´N, 0°47.87´W 14 60.98 0.201 0.190      
Tunisia
14 Cape Bon 37°04.27´N, 11°04.27´E 10 47.56 0.151 0.151      
Total   215 97.56     0.179 0.258 0.31
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Fig. 1. Location map and Bayesian analysis of the genetic structure of 215 individuals from fourteen sampled populations of T. articulata. Pie charts represent the mean proportion of membership to the predefined K = 2 clusters with the highest ΔK obtained following Evanno et al. (2005). Inverted triangles, Morocco populations; triangles, Algeria populations; grey square, Malta populations; dark diamond, Spain populations; open circle, Tunisia population.

Table 2. ISSR primers used to generate multilocus profiles with DNA from T. articulata.
Primer Sequence Range No. bands % polymorphic
OLIGO1 (GT)6RG 200–1220 20 100
OLIGO4 (GT)6AY 190–1310 12 100
UBC857 (AC)8YG 220–1405 18 100
UBC881 (GGGT)3G 200–1550 16 87.5
OW12 (GA)8YA 230–1330 16 100
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Fig. 2. UPGMA dendrogram constructed using pair-wise Nei’s (1973) genetic distance derived by ISSR analysis for populations of T. articulata.

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Fig. 3. Principal coordinate analysis of the 215 individuals from fourteen sampled populations of T. articulata based on pair-wise Nei’s (1973) genetic distances.