Table 1. RAPD primers used for fingerprinting bamboo species from a Brazilian collection.
Identification Sequence
OPC-20 5’-ACTTCGCCAC-3’
OPV-17 5’-ACCGGCTTGT-3’
OPG-17 5’-ACGACCGACA-3’
OPAN-03 5’-AGCCAGGCTG-3’
OPB-10 5’-CTGCTGGGAC-3’
OPAW-20 5’-TGTCCTAGCC-3’
OPAW-08 5’-CTGTCTGTGG-3’
OPJ-01 5’-CCCGGCATAA-3’
OPJ-15 5’-TGTAGCAGGG-3’
OPJ-04 5’-CCGAACACGG-3’
OPI-15 5’-TCATCCGAGG-3’
OPL-04 5’-GACTGCACAC-3’
Table 2. Total number of bands (N), species with unique bands (UB) and number of unique bands detected with RAPD and RAPD-RFLP markers in bamboo species.
Marker N UB PIC Unique bands per species
     RAPD BV BVV BB DG DA PE PH GA GS G1 G2 G3 G4
OPC-20 1 0 0.00
OPV-17 5 1 0.74 1
OPG-17 7 2 0.85 1 1
OPAN-03 7 1 0.71 1
OPB-10 10 2 0.78 1 1
OPAW-20 9 4 0.91 1 1 1 1
OPAW-08 5 1 0.88 1
OPJ-01 5 3 0.94 1 2
OPJ-15 6 1 0.56 1
OPJ-04 7 1 0.84 1
OPI-15 12 2 0.89 1 1
OPL-04 9 1 0.81 1
 Total 83 19 0.74 1 2 4 2 0 1 5 3 0 1 0 0 0
   RAPD-RFLP                          
MspI 33 9 0.86 2 4 1 2
HindIII/HaeIII 26 14 0.94 5 1 2 4 1 1
HinfI/RsaI 35 11 0.93     4     3       3     1
 Total 94 34 0.91 0 2 13 0 2 5 2 4 0 3 1 1 1
BV – Bambusa vulgaris. BVV – Bambusa vulgaris vittata. BB – Bambusa beecheyana. DG – Dendrocalamus giganteus. DA – Dendrocalamus asper. PE – Phyllostachys edulis. PH – Phyllostachys heterocycla. GA – Guadua amplexifolia. GS – Guadua superba. G1, G2, G3 and G4 – Guadua spp.

Fig. 1. RAPD profile of OPV-17 for 13 bamboo taxa. BVV – Bambusa vulgaris vittata. BV – Bambusa vulgaris. BB – Bambusa beecheyana. PE – Phyllostachys edulis. PH – Phyllostachys heterocycla. DA – Dendrocalamus asper. DG – Dendrocalamus giganteus. GA – Guadua amplexifolia. GS – Guadua superba. G1, G2, G3 and G4 – Guadua spp. M – 1 Kb Plus Ladder DNA.

Fig. 2. RAPD-RFLP profile from the digestion of RAPD products from primer OPV17 with three restriction enzyme combinations. BVV – Bambusa vulgaris vittata. BV – Bambusa vulgaris. BB – Bambusa beecheyana. DG – Dendrocalamus giganteus. DA – Dendrocalamus asper. PE – Phyllostachys edulis. PH – Phyllostachys heterocycla. GA – Guadua amplexifolia. GS – Guadua superba. G1, G2, G3 and G4 – Guadua spp. M – 100 bp marker (Ladder).

Fig. 3. Dendrogram of 13 bamboo taxa based on (a) RAPD and (b) RAPD-RFLP markers, and the (c) joint analysis. BVV – Bambusa vulgaris vittata. BV – Bambusa vulgaris. BB – Bambusa beecheyana. PE – Phyllostachys edulis. PH – Phyllostachys heterocycla. DA – Dendrocalamus asper. DG – Dendrocalamus giganteus. GA – Guadua amplexifolia. GS – Guadua superba. G1, G2, G3 and G4 – Guadua spp.

Table 3. Jaccard’s similarity coefficient among 13 bamboo taxa, based on 83 RAPD markers.
BV BVV BB DG DA PE PH GA GS G1 G2 G3 G4
BV 0.833 0.486 0.304 0.318 0.325 0.271 0.212 0.204 0.245 0.235 0.18 0.184
BVV 0.459 0.283 0.295 0.3 0.25 0.216 0.184 0.226 0.216 0.184 0.188
BB   0.372 0.39 0.3 0.304 0.265 0.234 0.226 0.216 0.208 0.213
DG   0.938 0.213 0.275 0.264 0.286 0.296 0.264 0.212 0.216
DA     0.222 0.286 0.275 0.298 0.308 0.275 0.22 0.224
PE       0.526 0.333 0.244 0.189 0.2 0.217 0.222
PH         0.447 0.28 0.268 0.259 0.255 0.26
GA           0.61 0.521 0.489 0.467 0.477
GS             0.683 0.692 0.55 0.564
G1               0.825 0.683 0.659
G2                 0.737 0.711
G3                   0.968
BV – Bambusa vulgaris. BVV – Bambusa vulgaris vittata. BB – Bambusa beecheyana. DG – Dendrocalamus giganteus. DA – Dendrocalamus asper. PE – Phyllostachys edulis. PH – Phyllostachys heterocycla. GA – Guadua amplexifolia. GS – Guadua superba. G1, G2, G3 and G4 – Guadua spp.
Table 4. Jaccard’s similarity coefficient among 13 bamboo taxa, based on 94 RAPD-RFLP markers.
BBV BV BB DG DA PE PH GA GS G1 G2 G3 G4
BBV 0.826 0.209 0.172 0.188 0.114 0.154 0.106 0.098 0.086 0.121 0.093 0.121
BV 0.238 0.172 0.188 0.14 0.154 0.13 0.098 0.086 0.121 0.119 0.121
BB   0.1 0.143 0.157 0.146 0.192 0.196 0.116 0.146 0.213 0.146
DG   0.765 0.025 0.028 0.023 0.057 0 0.036 0.054 0.036
DA     0.047 0.051 0.067 0.108 0.062 0.1 0.103 0.1
PE       0.677 0.311 0.3 0.098 0.128 0.227 0.1
PH         0.341 0.333 0.108 0.111 0.22 0.111
GA           0.571 0.371 0.382 0.541 0.382
GS             0.519 0.538 0.724 0.538
G1               0.65 0.433 0.65
G2                 0.5 0.778
G3                   0.556
BVV – Bambusa vulgaris vittata. BV – Bambusa vulgaris. BB – Bambusa beecheyana. DG – Dendrocalamus giganteus. DA – Dendrocalamus asper. PE – Phyllostachys edulis. PH – Phyllostachys heterocycla. GA – Guadua amplexifolia. GS – Guadua superba. G1, G2, G3 and G4 – Guadua spp.

Fig. 4. Principal coordinate analysis of 13 bamboo taxa based on (a) RAPD, (b) RAPD-RFLP and (c) the joint analysis. BVV – Bambusa vulgaris vittata. BV – Bambusa vulgaris. BB – Bambusa beecheyana. DG – Dendrocalamus giganteus. DA – Dendrocalamus asper. PE – Phyllostachys edulis. PH – Phyllostachys heterocycla. GA – Guadua amplexifolia. GS – Guadua superba. G1, G2, G3 and G4 – Guadua spp.