Fig. 1. A) Symptoms of Diplodia tip blight observed in the field on Scots pine seedling in August 2021, three-year-old seedling died due to the disease; B) Symptoms of Diplodia tip blight could be seen easily still in April 2022 in the field in young Scots pine (~15 years old); C) Symptoms of Diplodia tip blight recorded in November 2021 in 15 years old Scots pine.
Fig. 2. A) Mature Scots pine with Diplodia tip blight symptoms in the field, April 2022. B) The shoots were brown and dead needles were still attached to the tips.
| Table 1. Site locations in Finland, sequence ID in GeneBank and necrosis length of the representative Diplodia sapinea strains. | |||
| Location | Tree age status | Sequence ID in NCBI | Inoculation experiment, arithmetic necrosis length (cm) |
| Helsinki | mature | OP103745 | 1.03 |
| Borderline Helsinki-Sipoo | mature | OP103747 | 4.03 |
| Borderline Helsinki-Sipoo | sapling* | OP103746 | 1.57 |
| Vantaa | seedling | OP103744 | 1.60 |
| Uusikaupunki | mature | OP103748 | 2.00 |
| Naantali | mature | OP103743 | 2.03 |
| Naantali | seedling | OP103742 | 3.20 |
| * 15-year-old Scots pines | |||
Fig. 3. A) Disease symptoms of Scots pine seedling inoculated with Diplodia sapinea; B) Mock-control seedlings remained asymptomatic.