Table 1. Number of collected galls, number of necrotic galls and number of insects died as larval stage, pupa or adult within the necrotic galls and percentage of empty chambers from each collecting date in both chestnuts (C4 and C5).
Number of
collected galls 		Number of necrotic galls Nº of insect died inside the necrotic galls of chestnut C4 Nº of insects died inside the necrotic galls of chestnut C5 % of empty chambers in the necrotic galls
Date C4 C5 C4 C5 Larva Pupa Adult Total Larva Pupa Adult Total C4 C5
09-Jul-14 52 54 18 1 2 4 0 6 1 0 0 1 55.6 66.7
23-Jul-14 35 21 9 8 0 1 1 2 1 0 2 3 66.7 50.0
7-Aug-14 47 26 12 10 0 0 1 1 0 0 2 2 91.7 80.0
22-Aug-14 67 25 12 11 0 0 0 0 0 0 2 2 100.0 81.8
12-Sept-14 43 35 13 11 0 0 0 0 0 0 1 1 100.0 91.7
29-Sep-14 44 28 0 14 0 0 0 0 0 0 1 1 - 93.3
Total 288 189 64 55 2 5 2 9 2 0 8 10
Table 2. Fungal species isolated from the morphotypes of the necrotic galls.
Sample Month Tissue Fungal taxa Accession number
1 July Necrotic gall Pestalotiopsis sp. KU095868
2 July Necrotic gall Epicoccum nigrum KU095869
3 July Necrotic gall Epicoccum nigrum KU095870
4 July Necrotic gall Penicillium ramulosum KU095871
5 July Necrotic gall Epicoccum nigrum KU095872
6 August Necrotic gall Epicoccum nigrum KU095873
7 September Necrotic gall Epicoccum nigrum KU095874
8 September Necrotic gall Fusarium oxysporum KU095875
9 September Necrotic gall Gnomoniopsis smithogilvyi KU095876
10 September Necrotic gall Fusarium avenaceum KU095877
11 September Necrotic gall Fusarium sp. KU095878
Table 3. Relative abundance for each morphotype among the isolated cultures.
Morphotypes Fungal taxa Nº of isolates Relative abundance
1 Pestalotiopsis sp. 1 0.008
2 Epicoccum nigrum 1 0.008
3 Epicoccum sp. 18 0.144
4 Penicillium ramulosum 2 0.016
5 Epicoccum nigrum 9 0.072
6 Epicoccum nigrum 8 0.064
7 Epicoccum nigrum 12 0.096
8 Fusarium oxysporum 11 0.088
9 Gnomoniopsis smithogilvyi 25 0.20
10 Fusarium avenaceum 24 0.192
11 Fusarium sp. 14 0.112