1

Fig. 1. Sampling locations of fungal associates to Megaplatypus mutatus in Argentina. AP: Morse, Province of Buenos Aires- Populus deltoides; BP: Bragado, Province of Buenos Aires- Populus deltoides; CC: Concordia, Province of Entre Rios- Casuarina cunninghamiana.

Table 1. Location, Host plants and sample collection of fungi associated to Megaplatypus mutatus galleries in Argentina. Locations are named as City and Province, respectively.
Sample ID Location Host plant No. of
trees 		No. of
galleries 		Metagenomic
samples 		Collection
points
AP A. Morse
Buenos Aires 		Populus deltoides 2 2 2 90
BP B. Bragado
Buenos Aires 		Populus deltoides 2 2 2 90
CC C. Concordia
Entre Ríos 		Casuarina cunninghamiana 2 2 2 90
2

Fig. 2. Principal Coordinate Analysis plots of fungal communities according to the host plant order associated to Megaplatypus mutatus galleries. PCoA based on Bray–Curtis dissimilarities. Sampled locations in Argentina, AP: Morse, Buenos Aires- Populus deltoides; BP: Bragado, Buenos Aires- Populus deltoides; CC: Concordia, Entre Rios- Casuarina cunninghamiana.

Table 2. Taxonomic assignments of associated fungi to Megaplatypus mutatus galleries in three locations. Each column represents a different location and each row a taxonomic assignment. Sampled locations in Argentina, AP: Morse, Buenos Aires- Populus deltoides; BP: Bragado, Buenos Aires- Populus deltoides; CC: Concordia, Entre Rios- Casuarina cunninghamiana.
Total relative frequency per taxa (%) Taxonomic assignments Relative frequency per location (%)
AP BP CC
33.71 Candida insectalens 2.38 3.99 91.46
15.29 Paracremonium sp. 0.01 41.77 0
12.78 Phaeoisaria clematidis 0.01 34.92 0
12.22 Coprinellus radians 42.24 0 0.01
6.34 Dipodascacea 21.93 0.01 0
4.92 Chaetomium sp. 17.04 0 0
3.88 Fusarium sp. 1 0.01 3.05 7.98
3.45 Coprinellus micaceus 0 9.44 0
2.73 Dekkera sp. 9.44 0 0
1.98 Fusarium sp. 2 0.1 5.35 0
1.08 Paecilomyces sp. 3.76 0 0
0.70 Graphium sp. 2.43 0 0
0.52 Fusarium sp. 3 0.01 1.42 0
0.16 Arthrobotrys sp. 0.54 0 0
0.07 Kodamaea sp. 0 0 0.2
0.05 Fusarium oxysporum species complex 0 0 0.14
0.04 Clonostachys rosea 0 0 0.12
0.03 Candida sp. 1 0.1 0 0
0.03 Fellomyces sp. 0 0.01 0.07
0.02 Phialemonium dimorphosporum 0 0.04 0.02
100   100 100 100
Table 3. Diversity and richness indexes from associated fungi to Megaplatypus mutatus galleries. Calculated from locations-dataset. Diversity indexes show the highest values for CC sample. The most similar set of samples was AP-BP. Sampled locations in Argentina were, AP: Morse, Buenos Aires- Populus deltoides; BP: Bragado, Buenos Aires- Populus deltoides; CC: Concordia, Entre Rios- Casuarina cunninghamiana.
Sample ID ACE Chao1 Shannon Simpson 1° Sample 2° Sample Shared Taxa Sorensen
AP 11.52 10.99 1.07 2.65 AP BP 7 0.58
BP 17.14 16.92 1.74 4.56 AP CC 3 0.27
CC 20 20 2.05 5.59 BP CC 4 0.44