Table 1. The location, code, and percentage of females in the studied populations and the number of male and female Taxus baccata individuals analysed.
Population Pop. Number Longitude/Latitude % females a Analyzed
males
Analyzed
females
Leszno 1 53°46´N/20°52´E 45 50 50
Choczewskie Cisy 2 54°46´N/17°45´E 47 36 46
Cisy nad Czerską Strugą 3 53°45´N/17°57´E 46 40 47
Jelenia Góra 4 53°35´N/18°06´E 50 38 36
Wierzchlas 5 53°30´N/18°06´E 33 50 50
Cisy w Czarnem 6 53°44´N/16°58´E 49 48 48
Cisy Rokickie 7 53°45´N/14°51´E 64 49 50
Nadleśnictwo Rokita 8 53°42´N/14°51´E 63 25 46
Boleszkowice 9 52°42´N/14°33´E 55 37 55
Bogdaniec 10 52°41´N/15°04´E 32 32 32
Książ 11 50°50´N/16°16´E 37 43 37
Cisowa Góra 12 50°32´N/16°42´E 53 50 50
Cisy koło Barda 13 50°32´N/16°39´E 51 50 50
Nowy Waliszów 14 50°18´N/16°45´E 51 50 50
Cisy nad Liswartą 15 50°44´N/18°46´E 54 34 55
Cisy w Hucie Starej 16 50°33´N/19°12´E 48 26 27
Jasień 17 50°59´N/19°33´E 55 41 52
Radomice 18 50°44´N/20°39´E 68 29 65
Mogilno 19 49°39´N/20°49´E 58 38 53
Wadernik 20 49°29´N/21°38´E 51 26 30
Igiełki 21 49°30´N/21°38´E 54 30 39
Kretówki 22 49°44´N/21°53´E 40 58 39
Malinówka 23 49°41´N/21°55´E 44 50 50
Pasmo Łysej Góry 24 49°33´N/21°34´E 55 42 41
Serednica 25 49°28´N/22°31´E 46 31 26
Cisy na Górze Jawor 26 49°17´N/22°17´E 55 38 34
a Based on data collected by Kostrzyca Forest Gene Bank
1

Fig. 1. Map of the geographical locations of the 26 Taxus baccata populations included in this study. Codes representing the named populations are listed in Table 1, and the borders of the natural T. baccata distribution in Poland are indicated with a black line.

Table 2. Parameters used to estimate the genetic structure of the studied Taxus baccata populations averaged across five nSSR loci. A – average number of alleles, AR25 –allelic richness, He – expected heterozygosity, Ho – observed heterozygosity, N0 – null allele frequency. View in new window/tab.
Table 3. Parameters used to estimate the level of genetic diversity and differentiation in male and female individuals A – average number of alleles, AR25 – allelic richness, He – expected heterozygosity, Ho – observed heterozygosity, N0 – null allele frequency, Fst –genetic differentiation, p–values of the statistical significance of differences between the two sample groups.
Parameter Female samples Male samples p-value
A 9.512 9.469 0.945
AR25 7.769 7.744 0.965
He 0.730 0.731 0.911
Ho 0.479 0.487 0.876
N0 0.146 0.137 0.275
Fst 0.149 0.152 0.901
2

Fig. 2. Results of Principal Component Analysis based on five nuclear SSR markers in male and female Taxus baccata individuals.