Leena Ryynänen , Martti Ryynänen

Propagation of adult curly-birch succeeds with tissue culture.

Ryynänen L., Ryynänen M. (1986). Propagation of adult curly-birch succeeds with tissue culture. Silva Fennica vol. 20 no. 2 article id 5269. https://doi.org/10.14214/sf.a15448

Abstract

The curly-grained trait of Betula pendula Roth is inheritable, but it is persumably not question of only one Mendelian gene since, for instance, there are a number of different types of curly-birch. The progeny obtained from controlled crossing between two curly-birch individuals do not all posses the curly-grained trait.

Plantlets were produced from adult curly-birch (Betula pendula var. carelica). Murashige and Skoog’s medium was used as the culture medium. Growth was initiated on a medium containing 1 mg/l BAP. Bud formation was induced using a medium containing 10 mg/l BAP and 0.2 mg/l NAA. Development of shoots was achieved on a medium containing ½ x Murashige and Skoog’s macrominerals and sucrose, 1/1 x Murashige and Skoog’s microminerals and vitamins, and 0.5 mg/l BAP and 0.5 mg/l IAA. The medium used for inducing root formation was the same as above, but without any growth regulators. The results indicate that adult deciduous trees can be best propagated through tissue culture when the least differentiated cells, i.e. the initial cells of the promeristem, are used as the startin material. The axillary buds provide easily available study material which can be prepared with little difficulty and are continuously renewed.

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Keywords
cell differentiation; Betula pendula var. carelica; tissue culture; clonal propagation; clonal variegation; plantlets

Published in 1986

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Available at https://doi.org/10.14214/sf.a15448 | Download PDF