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Articles containing the keyword 'direct PCR'

Category : Research note

article id 7749, category Research note
Eeva J. Vainio, Sannakajsa M. Velmala, Pertti Salo, Seppo Huhtinen, Michael M. Müller. (2017). Defoliation of Tilia cordata trees associated with Apiognomonia errabunda infection in Finland. Silva Fennica vol. 51 no. 4 article id 7749. https://doi.org/10.14214/sf.7749
Keywords: small-leaved lime; fungal disease; anthracnose; ITS rDNA; direct PCR; Tiliaceae
Highlights: Defoliation of Tilia cordata was investigated by fungal isolation from symptomatic leaf petioles and ITS sequence determination; The disease symptoms were associated with the presence of Apiognomonia errabunda; We report the first nucleotide sequences of A. errabunda from the Nordic countries.
Abstract | Full text in HTML | Full text in PDF | Author Info

We investigated the causative agent of a disease outbreak affecting small-leaved limes (Tilia cordata Mill.) and resulting in darkening of the leaf petioles and excessive defoliation during summer 2016 in southern Finland. The fungal species composition of the symptomatic petioles was examined by culture isolation and molecular identification using ITS rDNA sequences, which revealed the most prevalent fungal species present in the petioles as Apiognomonia errabunda (Roberge) Höhn. Based on reviewing curated herbarium specimens deposited at the Universities of Helsinki and Turku, A. errabunda is native and widely distributed in small-leaved limes in Finland, and occasionally infects also other broadleaved trees, including Quercus robur L. and ornamental species of Tilia L. and Fagus L. The ITS sequence analysis conducted during this study revealed minor within-species polymorphisms similar to those observed earlier in the Central European and Russian populations of A. errabunda, and reports the first nucleotide sequences of this species from the Nordic countries.

  • Vainio, Natural Resources Institute Finland (Luke), Management and Production of Renewable Resources, P.O. Box 2, FI-00791 Helsinki, Finland ORCID http://orcid.org/0000-0002-6739-7968 E-mail: eeva.vainio@luke.fi (email)
  • Velmala, Natural Resources Institute Finland (Luke), Management and Production of Renewable Resources, P.O. Box 2, FI-00791 Helsinki, Finland E-mail: sannakajsa.velmala@luke.fi
  • Salo, Finnish Museum of Natural History, Botanical Museum, P.O. Box 7, FI-00014 University of Helsinki, Finland E-mail: pertti.salo@helsinki.fi
  • Huhtinen, University of Turku, Herbarium, Biodiversity Unit, FI-20014 Turku, Finland E-mail: sephuh@utu.fi
  • Müller, Natural Resources Institute Finland (Luke), Management and Production of Renewable Resources, P.O. Box 2, FI-00791 Helsinki, Finland E-mail: micms.muller@gmail.com

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