Seedlings from four Norway spruce (Picea abies (L.) H. Karst.) stands originating from areas with effective temperature sums ranging from 710 d.d. to 1,150 d.d. were raised under artificial light and temperature treatment. After a 10-week growing period the hardening process was started by subjecting the seedlings to +8°C night temperature and +15°C day temperature, and increasing the night length by 1.5 hour/week. Hardiness was measured by means of artificial freezing treatment (-10°C or -15°C), followed by visual estimation of the degree of needle injury. The stem height, lignification and bud development were measured before the freezing treatment. The amount of injury increased the more southern the origin of the tested material was. Furthermore, the proportion of non-lignified part of the seedling stem was negatively correlated with the latitude of the provenances. The proportion of seedlings with clearly visible buds was more than 90% in the northernmost entry and less than 1% in the southernmost one. The overall correlation coefficient between the needle injuries and the proportion of non-lignified part of the stem was rather high, but varied considerably from 0.3 in the northernmost material to over 0.6 in the southern provenances. According to the results, it seems to be possible to use growth characteristics as an indicator of frost hardiness at the provenance level.
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Saprotrophic wood-decaying abilities of Rigidoporus microporus (Polyporales, Basidiomycota) syn. Rigidoporus lignosus and the structural alterations induced in wood blocks of Hevea brasiliensis Muell. Arg were studied. Mass loss of wood blocks was analyzed after 3 and 6 months respectively and the patterns of decay by pathogenic and endophytic isolates of this fungus were investigated using light microscopy. Effects of temperature on growth of the isolates on malt extract agar were also investigated. The R. microporus isolated from a non-H. brasiliensis host caused the highest percentage mass loss (27.2% after 6 months), followed by isolates ED310 (21.1%) and M13 (15.7%), both collected from diseased H. brasiliensis plantations. The isolate initially identified as an endophyte showed very low saprotrophic wood decay capability (4.3% after 6 months). The optimal temperature for growth of the isolates was 30 °C; except for the endophytic isolate which showed highest growth at 25 °C. Wood samples degraded by the R. microporus isolates showed simultaneous attack of wood cell walls, typical of white rot fungi. Results of the study indicate variability in the wood degrading abilities of the isolates and the potential differences in their physiology are discussed. Our findings further support the need for a taxonomical revision of the Rigidoporus genus.