Pablo Martínez-Álvarez, Fernando Manuel Alves-Santos, Julio Javier Diez. (2012). In vitro and in vivo interactions between Trichoderma viride and Fusarium circinatum. Silva Fennica vol. 46 no. 3 article id 42. https://doi.org/10.14214/sf.42

Keywords: pitch canker; antagonism; biocontrol; biological control agents; endophytes; Pinus radiata; Spain

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Fusarium circinatum, a fungus that causes pitch canker disease, has been present in Europe since at least 2003, when it was detected in northern Spain and found to be producing severe damage in tree nurseries and pine plantations. In this study, we tested a method of biological control of the disease with Trichoderma viride, a fungal species successfully used against many other pathogens. In vitro and in vivo assays were carried out to test the efficacy of this antagonist in controlling F. circinatum. The T. viride isolate exerted a significant effect on the growth of F. circinatum in the in vitro assay, reducing the length of the pathogen colony by half. However, although we tested three different concentrations of the T. viride spore solution, no clear conclusions were obtained with regard to the effects on the Pinus radiata seedlings. To our knowledge, this is the first study carried out with the aim of using Trichoderma spp. to control pitch canker disease.

Martínez-Álvarez, Sustainable Forest Management Research Institute, University of Valladolid – INIA, Avenida de Madrid 44, 34071 Palencia, Spain E-mail: pmtnez@pvs.uva.es
Alves-Santos, Sustainable Forest Management Research Institute, University of Valladolid – INIA, Avenida de Madrid 44, 34071 Palencia, Spain E-mail: fmas@nn.es
Diez, Sustainable Forest Management Research Institute, University of Valladolid – INIA, Avenida de Madrid 44, 34071 Palencia, Spain E-mail: jjd@nn.es

article id 40, category Research article

Jorge Martín-García, Elba Espiga, Valentín Pando, Julio Javier Diez. (2011). Factors influencing endophytic communities in poplar plantations. Silva Fennica vol. 45 no. 2 article id 40. https://doi.org/10.14214/sf.40

Keywords: endophyte; poplar; management; site quality; foliar nutrients; forest health

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The fungal species associated with leaves and twigs from stands of Populus x euramericana in northern Spain were studied with the aim of evaluating the effects of several factors on endophytic communities in these plantations. Endophyte assemblages were analysed in 12 poplar plantations (clone I-214), chosen according to a factorial scheme with two factors: age and site quality. Crown condition, dendrometric variables and foliar nutrients were recorded in each sampled tree to evaluate their effects on endophytic communities. Fungal species richness and relative isolation frequency (RIF) were higher in young stands than in adult stands. Moreover, the age-related differences depended on site quality, with the lowest richness levels observed in adult stands located in poor sites. At stand level, endophyte assemblages varied among stands according to site quality and, to a lesser extent, stand age. On the other hand, crown discoloration, total height and foliar concentrations of iron and zinc may be key indicators of endophytic communities in poplar plantations, at tree level.

Martín-García, Sustainable Forest Management Research Institute, University of Valladolid – INIA, Avenida de Madrid 57, 34004 Palencia, Spain, and Forestry Engineering, University of Extremadura, Plasencia, Spain E-mail: jorgemg@pvs.uva.es
Espiga, Sustainable Forest Management Research Institute, University of Valladolid – INIA, Palencia, Spain E-mail: ee@nn.es
Pando, Statistics and Operations Research Department, University of Valladolid, Spain E-mail: vp@nn.es
Diez, Sustainable Forest Management Research Institute, University of Valladolid – INIA, Palencia, Spain E-mail: jjd@nn.es

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