Nadia Goué, Nathalie Noël-Boizot, Michel Vallance, Elisabeth Magel, Philippe Label. (2012). Microdissection to isolate vascular cambium cells in poplar. Silva Fennica vol. 46 no. 1 article id 62. https://doi.org/10.14214/sf.62

Keywords: fusiform cambial cells; microgenomics; Populus spp.; ray cambial cells

Abstract | View details | Full text in PDF | Author Info

Vascular cambium is the lateral meristem producing xylem cells inwards and phloem cells outwards in plant stem. Thus, in trees, the quality and quantity of wood is a result of highly regulated developmental process depending initially on the vascular cambium cell production. The availability of accurate transcriptomics technologies based on high coverage sequencing raises the level of expectations on tissue sampling to a very high degree. What is the benefit of top-level transcriptomics in wood formation studies if we are using these technologies on raw tissues, mixing cells at the organ level or even higher scale? The presented work describes a nine-step procedure, from standing tree to isolated ray and fusiform cells from cryolyophilized tangential sections of the poplar cambial zone. The aim of this paper is to present a step by step procedure including advices on how to select the optimal tree, how to fell the tree while securing its physiological parameters, how to cryolyophilize and microdissect under binocular, presenting the time schedule of the whole process and RNA analysis.

Goué, UMR5546 CNRS-Université Paul Sabatier, Castanet Tolosan, France; (INRA-UAGPF, 2163 Avenue de la Pomme de Pin, 45075 Orléans cedex 2, France) E-mail: goue@lrsv.ups-tlse.fr
Noël-Boizot, INRA-UAGPF, 2163 Avenue de la Pomme de Pin, 45075 Orléans cedex 2, France E-mail: nnb@nn.fr
Vallance, INRA-UAGPF, 2163 Avenue de la Pomme de Pin, 45075 Orléans cedex 2, France E-mail: mv@nn.fr
Magel, Universität Hamburg, Zentrum Holzwirtschaft, Deutschland E-mail: em@nn.de
Label, INRA-UAGPF, 2163 Avenue de la Pomme de Pin, 45075 Orléans cedex 2, France E-mail: pl@nn.fr

Category : Research note

article id 1126, category Research note

Rebecka Mc Carthy, Per Magnus Ekö, Lars Rytter. (2014). Reliability of stump sprouting as a regeneration method for poplars: clonal behavior in survival, sprout straightness and growth. Silva Fennica vol. 48 no. 3 article id 1126. https://doi.org/10.14214/sf.1126

Keywords: Populus spp.; coppice regeneration; vegetative regeneration; short-rotation forestry

Highlights: Clonal differences were found in stump sprout survival; Clonal differences occurred in sprout straightness; It is uncertain if the ability to produce a high number of stump sprouts is beneficial.

Abstract | Full text in HTML | Full text in PDF | Author Info

The interest in poplars (Populus spp.) has increased globally as a result of the current incentive to expand the use of biomass for energy. So far, knowledge about poplar stump sprouting is generally poor. The objective of this study was to investigate poplar stump sprouting as regeneration method in harvested plantations. A 19-year-old poplar clonal test, including 23 different clones, on former arable land was harvested in the winter. After one year, stumps were assessed for sprout survival, sprout straightness, number of sprouts, sprout height and basal diameter. Differences between clones were found for survival and sprout straightness. Clonal differences were also found in mean size of sprouts (basal diameter and height). However, no differences were found between clones when comparing size of the tallest sprout or number of sprouts produced on stumps. This study indicates that stump sprouts from various poplar clones differ in survival, sprout straightness and mean growth, which should be taken into account when planning for the next tree generation. The study needs to be complemented with other studies to better assess sprouting characteristics for different clones.

Mc Carthy, Forestry Research Institute of Sweden, Skogforsk, Ekebo 2250, SE-268 90 Svalöv, Sweden & Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences, P.O. Box 49, SE-230 53 Alnarp, Sweden E-mail: rebecka.mccarthy@skogforsk.se
Ekö, Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences, P.O. Box 49, SE-230 53 Alnarp, Sweden E-mail: Per.Magnus.Eko@slu.se
Rytter, Forestry Research Institute of Sweden, Skogforsk, Ekebo 2250, SE-268 90 Svalöv, Sweden E-mail: lars.rytter@skogforsk.se

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