Articles containing the keyword 'colchicine'

Seeds of ten different Betula species were treated with colchicine solution during germination, to induce duplication of the chromosome set. The species included in the study were B. pendula, B. pubescens, B. papyrifera subcordata, B. papyrifera papyrifera, B. papyrifera occidentalis, B. populifolia, B. alleghaniensis, B.n igra, B. glandulosa and B. nana. The total number of individually labelled, colchicine-treated trees was 1,550. Colchicine treatment induced changes in morphological features, especially in the leaves. These features proved to be good indicators of polyploidization. The experiments produced 687 polyploid trees, 287 of which are still alive. The polyploid Betula trees offer possibilities of studying the significance of the genome dosage for the growth, breeding, adaptability and evolution of Betula.

The PDF includes an abstract in Finnish.

• Särkilahti, E-mail: es@mm.unknown
• Valanne, E-mail: tv@mm.unknown

Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination in vitro and crossing induced pollen with normal gametes to produce triploid in Populus tomentosa Carrière. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed (p < 0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce P. tomentosa to produce 2n pollen and will not lead to dysfunction of induced diploid pollen.

• Liu, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: 342767649@qq.com
• Zhang, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: 409192881@qq.com
• Zhou, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: 876034493@qq.com
• Wu, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: 1269485709@qq.com
• Zhang, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: zhangpd@bjfu.edu.cn

• Wang, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: wangjun@bjfu.edu.cn
• Shi, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: 7320932@qq.com
• Song, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: angela-song@foxmail.com
• Tian, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: 1215813245@qq.com
• Kang, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: kangxy@bjfu.edu.cn