Articles containing the keyword 'molecular markers'

Vaccinium myrtillus L., V. vitis-idaea L. and V. uliginosum L. belong to the genus Vaccinium. These wild species are widely distributed and ecologically important within the Baltic countries but they have not been extensively studied using molecular markers. EST-SSR and cpSSR markers were used to investigate the population structure and genetic diversity of these species to obtain information useful for the development of in situ conservation strategies. Wild Vaccinium species populations are moderately genetically differentiated, with some populations more highly differentiated, but without higher order clustering of groups of populations, indicating that there are no dispersal barriers for these species within the Baltic countries. Genetic diversity of populations growing in protected areas, managed forests and intensively utilised public recreational areas is similar. The results from this study can be utilised for the selection of populations for the in situ conservation of the studied Vaccinium species. In addition, complementary ex situ conservation strategies can be used for the preservation of rare varieties (e.g. V. myrtillus var. leucocarpum).

• Gailīte, Genetic Resource Centre, Latvian State Forest Research Institute “Silava”, 111 Rigas Street, Salaspils, Latvia, LV-2169 E-mail: agnese.gailite@silava.lv
• Gaile, Genetic Resource Centre, Latvian State Forest Research Institute “Silava”, 111 Rigas Street, Salaspils, Latvia, LV-2169 E-mail: anita.gaile@silava.lv
• Ruņģis, Genetic Resource Centre, Latvian State Forest Research Institute “Silava”, 111 Rigas Street, Salaspils, Latvia, LV-2169 https://orcid.org/0000-0001-5173-2912 E-mail: dainis.rungis@silava.lv

Bamboo species have a very significant ecological and economic impact. Determining morphological and genetic differences among bamboo genera and species are crucial to explore desirable traits for breeding purposes. Several advances have been made in the taxonomy of bamboos by using molecular fingerprinting tools and next generation sequencing technologies. Nevertheless, classical molecular markers such as RAPD (Random Amplified Polymorphic DNA), AFLP (Amplified Fragment Length Polymorphism) and ISSR (Inter Simple Sequence Repeats) also provide an accurate discrimination among genera and species. Moreover, the RAPD-RFLP (Random Amplified Polymorphic DNA, Restriction Fragment Length Polymorphism) method, in which amplification products from RAPD are digested with restriction enzymes, is a reliable, fast and cost-effective method for fingerprinting. RAPD-RFLP has been scarcely used in the literature and no report regarding bamboo taxonomy is available with this method. Here we explored the molecular (RAPD, RAPD-RFLP) variation among genera (Bambusa, Dendrocalamus, Guadua and Phyllostachys) and species of bamboo cultivated in Brazil. Both molecular markers allowed clear distinction among the genera studied. Moreover, high cophenetic correlation values in UPGMA clusters indicated their potential for discriminating bamboo species. The digestion of RAPD products (RFLP) resulted in high number of polymorphic bands and produced very characteristic profiles for each genus with three enzyme combinations (HindIII/HaeIII, HinfI/RsaI, and single digestion with MspI). We recommend RAPD-RFLP as a reproducible and informative method for screening differences among genera, species and varieties of bamboos. Providing a cost-effective and accurate method for species identification and characterization is straightforward for bamboo conservation, management and breeding.

• Konzen, Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of Sao Paulo (USP), Centenário Av., 303, Piracicaba, SP, P.O. Box 96, Brazil; Forest Sciences, Federal University of Lavras (UFLA), Lavras, MG, P.O. Box 3037, Brazil E-mail: erkonzen@gmail.com
• Peron, Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of Sao Paulo (USP), Centenário Av., 303, Piracicaba, SP, P.O. Box 96, Brazil E-mail: rperon@purdue.edu
• Ito, Embrapa Western Agriculture, Brazilian Agricultural Research Corporation (EMBRAPA), BR 163 Rd., km 253, Dourados, MS, P.O. Box 449, Brazil E-mail: marcio.ito@embrapa.br
• Brondani, Forest Sciences, Federal University of Lavras (UFLA), Lavras, MG, P.O. Box 3037, Brazil E-mail: gebrondani@gmail.com
• Tsai, Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of Sao Paulo (USP), Centenário Av., 303, Piracicaba, SP, P.O. Box 96, Brazil E-mail: tsai@cena.usp.br

• Androsiuk, University of Warmia and Mazury in Olsztyn, Department of Genetics, Plac Lodzki 3, 10-967 Olsztyn, Poland E-mail: pa@nn.pl
• Zielinski, University of Warmia and Mazury in Olsztyn, Department of Genetics, Plac Lodzki 3, 10-967 Olsztyn, Poland E-mail: rz@nn.pl
• Polok, University of Warmia and Mazury in Olsztyn, Department of Genetics, Plac Lodzki 3, 10-967 Olsztyn, Poland E-mail: kpolok@moskit.uwm.edu.pl

• Monteleone, DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy E-mail: im@nn.it
• Ferrazzini, DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy E-mail: df@nn.it
• Belletti, DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy E-mail: piero.belletti@unito.it