Current issue: 58(5)
Articles containing the keyword 'genetic diversity'
Category : Research article
Oceanic archipelagos provide an important platform from which to evaluate the effects of isolation and fragmentation on the genetic structure of species. As a result of oceanic isolation, such species usually show lower levels of genetic diversity and higher genetic differentiation than their mainland congeners. However, this is not necessarily the case for long distance dispersal species, whose genetic structure is not strictly defined by population isolation. We assessed the level and distribution of genetic diversity among Canarian populations of Juniperus turbinata in order to evaluate the influence of population isolation on its genetic structure. Using Amplified Fragment Length Polymorphism markers, we analyzed molecular diversity among 175 individuals from five populations occurring across the Canary Island and three Moroccan populations. Principal Coordinate Analysis, neighbor joining clustering, AMOVA and Bayesian-based analysis were applied to examine population structure. Despite the documented habitat loss and decline in Canarian populations, Amplified Fragment Length Polymorphism markers revealed levels of intra-population genetic diversity that were similar to those from mainland populations, and low levels of genetic differentiation. Bayesian analysis of population structure showed three main clusters, one comprising El Hierro population and a few individuals from several islands, a second cluster that grouped the remaining Canarian populations together, and a third cluster grouping Moroccan populations. Our results suggest that the main force driving the genetic structure of Canarian populations of J. turbinata is its capacity for long distance dispersal.
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Jimenez,
Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain
E-mail:
fjimenez@um.es
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Sánchez-Gómez,
Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain
http://orcid.org/0000-0001-6754-1512
E-mail:
psgomez@um.es
- Cánovas, Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain E-mail: joseluis.canovas@um.es
- Hensen, Institut für Biologie, Martin-Luther Universitat, 06099 Halle, Germany E-mail: isabell.hensen@botanik.uni-halle.de
- Aouissat, Centre Universitaire Salhi Ahmed Naama, BP 66, Naâma, Algérie E-mail: aouissatm@yahoo.fr
Populations at species’ range margins are expected to show lower genetic diversity than populations at the core of the range. Yet, long-lived, widespread tree species are expected to be resistant to genetic impoverishment, thus showing comparatively high genetic diversity within populations and low differentiation among populations. Here, we study the distribution of genetic variation in the pedunculate oak (Quercus robur L.) at its range margin in Finland at two hierarchical scales using 15 microsatellite loci. At a regional scale, we compared variation within versus among three oak populations. At a landscape scale, we examined genetic structuring within one of these populations, growing on an island of ca 5 km2. As expected, we found the majority of genetic variation in Q. robur to occur within populations. Nonetheless, differentiation among populations was markedly high (FST = 0.12) compared with values reported for populations of Q. robur closer to the core of its range. At the landscape level, some spatial and temporal sub-structuring was observed, likely explained by the history of land-use on the island. Overall, Q. robur fulfils the expectation of the central-marginal hypothesis of high differentiation among marginal populations, but the notable population differentiation has most likely been influenced also by the long, ongoing fragmentation of populations. Finnish oak populations may still be adjusting to the drastic habitat changes of the past centuries. Preservation of genetic variation within the remaining stands is thus an important factor in the conservation of Q. robur at its range margin.
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Pohjanmies,
University of Helsinki, Department of Agricultural Sciences, Spatial Foodweb Ecology Group, P.O. Box 27, FI-00014 University of Helsinki, Finland; University of Jyväskylä, Department of Biological and Environmental Sciences, P.O. Box 35, FI-40014 University of Jyväskylä, Finland
E-mail:
tahti.t.pohjanmies@jyu.fi
- Elshibli, Natural Resources Institute Finland (Luke), Green technology, P.O. Box 18, FI-01301 Vantaa, Finland; University of Helsinki, Department of Agricultural Sciences, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: sakina.elshibli@helsinki.fi
- Pulkkinen, Natural Resources Institute Finland (Luke), Green technology, Haapastensyrjäntie 34, FI-12600 Läyliäinen, Finland E-mail: pertti.pulkkinen@luke.fi
- Rusanen, Natural Resources Institute Finland (Luke), Green technology, P.O. Box 18, FI-01301 Vantaa, Finland E-mail: mari.rusanen@luke.fi
- Vakkari, Natural Resources Institute Finland (Luke), Green technology, P.O. Box 18, FI-01301 Vantaa, Finland E-mail: pekka.vakkari@luke.fi
- Korpelainen, University of Helsinki, Department of Agricultural Sciences, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: helena.korpelainen@helsinki.fi
- Roslin, University of Helsinki, Department of Agricultural Sciences, Spatial Foodweb Ecology Group, P.O. Box 27, FI-00014 University of Helsinki, Finland; Swedish University of Agricultural Sciences, Department of Ecology, P.O. Box 7044, SE-750 07 Uppsala, Sweden E-mail: tomas.roslin@helsinki.fi
English yew (Taxus baccata L.) is a strictly outcrossing and dioecious species whose populations are small and isolated. It is known that sex ratios may vary in natural populations due to local environmental conditions or stochastic events. However, unbalanced sex ratios may have negative impacts on genetic diversity through enhanced genetic drift and inbreeding. The present study represents one of the first attempts to compare the genetic variation at microsatellite loci within and between populations with different gender proportions. Our results indicated that there were no significant correlations between sex ratio and the extent of genetic variation in different populations. All populations exhibited high levels of genetic diversity. Additionally, the genetic structure was characterized separately in male and female individuals. Statistical analyses of the set estimators describing the genetic structure of male and female individuals of T. baccata revealed no significant differences between the two groups. Molecular analysis verified that microsatellite nuclear loci neutrality developed for T. baccata, as there were no significant differences in the genetic variation between males and females and no evidence for any outlier loci using coalescent and hierarchical Bayesian simulations. The results demonstrate that ignoring biased sex ratios in T. baccata populations had no effect on the assessment of genetic differentiation and genetic diversity within and between populations of this species. These results are discussed with regards to the practical application of molecular markers in conservation programs.
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Litkowiec,
Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland
E-mail:
mlit@man.poznan.pl
- Plitta-Michalak, Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland E-mail: beata-plitta@wp.pl
- Lewandowski, Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland E-mail: alew@man.poznan.pl
- Iszkuło, Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland & University of Zielona Góra, Faculty of Biological Sciences, Prof. Z. Szafrana 1, 65-516 Zielona Góra, Poland E-mail: iszkulo@man.poznan.pl
- Sánchez-Gómez, Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain E-mail: psgomez@um.es
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Jiménez,
Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain
E-mail:
fjimenez@um.es
- Vera, Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain E-mail: jbveraperez@gmail.com
- Sánchez-Saorín, Dirección General de Medio Ambiente, Consejería de Presidencia de la Región de Murcia, C/ Catedrático Eugenio Úbeda nº3, E-30071 Murcia, Spain E-mail: fjavier.sanchez3@carm.es
- Martínez, Dirección General de Medio Ambiente, Consejería de Presidencia de la Región de Murcia, C/ Catedrático Eugenio Úbeda nº3, E-30071 Murcia, Spain E-mail: juanf.martinez@carm.es
- Buhagiar, Argotti Herbarium and Gardens (UOM), Triq Vincenzo Bugeja Floriana VLT 16, Malta E-mail: joseph.buhagiar@um.edu.mt
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Jadwiszczak,
Institute of Biology, University of Białystok, wierkowa 20B, 15-950 Białystok, Poland
E-mail:
kszalaj@uwb.edu.pl
- Drzymulska, Institute of Biology, University of Białystok, wierkowa 20B, 15-950 Białystok, Poland E-mail: dd@nn.pl
- Banaszek, Institute of Biology, University of Białystok, wierkowa 20B, 15-950 Białystok, Poland E-mail: ab@nn.pl
- Jadwiszczak, Institute of Biology, University of Białystok, wierkowa 20B, 15-950 Białystok, Poland E-mail: pj@nn.pl
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Martín,
Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos de Madrid, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain
E-mail:
mariacarmen.martin@upm.es
- Parra, Departamento de Ciencias y Recursos Agrícolas y Forestales, Universidad de Córdoba, Avda. Linneo s/n, 14004-Córdoba, Spain E-mail: tp@nn.es
- Clemente-Muñoz, Departamento de Ciencias y Recursos Agrícolas y Forestales, Universidad de Córdoba, Avda. Linneo s/n, 14004-Córdoba, Spain E-mail: mcm@nn.es
- Hernández-Bermejo, Departamento de Ciencias y Recursos Agrícolas y Forestales, Universidad de Córdoba, Avda. Linneo s/n, 14004-Córdoba, Spain E-mail: ehb@nn.es
- Wang, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, China E-mail: yw@nn.cn
- Korpelainen, Department of Applied Biology, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: hk@nn.fi
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Li,
Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, China
E-mail:
licy@cib.ac.cn
- Monteleone, DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy E-mail: im@nn.it
- Ferrazzini, DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy E-mail: df@nn.it
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Belletti,
DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy
E-mail:
piero.belletti@unito.it
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Andersson,
Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden
E-mail:
erik.andersson@genfys.slu.se
- Spanos, N.AG.RE.F.-Forest Research Institute, 57006 Vassilika, Thessaloniki, Greece E-mail: kas@nn.gr
- Mullin, Genesis Forest Science Canada Inc., C.P. 64 Succursale Haute-Ville, Québec, QC G1R 4M8 Canada E-mail: tjm@nn.ca
- Lindgren, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden E-mail: dl@nn.se
Category : Research note
Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Đorđević; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is – exceptionally high levels of genetic diversity (HT = 0.949) and low genetic differentiation (GST = 0.024) at the cpDNA level, and moderate levels of genetic diversity (HT = 0.357) and genetic differentiation (GST = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans’ and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.
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Šarac,
University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology, Višegradska 33, 18000 Niš, Serbia
E-mail:
saraczorica@gmail.com
- Dodoš, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: tanjadodos@bio.bg.ac.rs
- Rajčević, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: nemanja@bio.bg.ac.rs
- Bojović, University of Belgrade, Institute for Biological Research “Siniša Stanković”, Boulevard Despota Stefana 142, 11060 Belgrade, Serbia E-mail: bojovic@ibiss.bg.ac.rs
- Marin, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: pdmarin@bio.bg.ac.rs
- Aleksić, University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11000 Belgrade, Serbia E-mail: aleksic_jelena@yahoo.com.au
