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Articles containing the keyword 'vegetative propagation'

Category : Article

article id 5466, category Article
Hely Häggman. (1991). Application of biotechnology to forest tree breeding. Silva Fennica vol. 25 no. 4 article id 5466. https://doi.org/10.14214/sf.a15624
Keywords: tree breeding; micropropagation; vegetative propagation; somatic embryogenesis; conventional breeding; somaclonal variation; biotechnology; gene transfer
Abstract | View details | Full text in PDF | Author Info

Forest tree breeding involves manipulation of genetic composition of populations and individuals, and biotechnology focuses on selected individuals. The new techniques cannot replace the conventional breeding techniques but both need effective cooperation of each other. Thus, the distinction between conventional breeding and biotechnology is artificial. The biotechnology methods are new and fast developing and the future with field and progeny testing will show which techniques will be permanently adopted into tree breeding. For instance, the earlier hope of the use of somaclonal variation as a new source of variability and a powerful tool for the breeder seem today quite the opposite. Somaclonal variation constituting a major problem in present-day micropropagation is due to the unpredictable variation. Based on knowledge of today, especially micropropagation via somatic embryos, transgenic trees and the identification of major genes seem to be good candidates to be permanently adopted into tree breeding.

  • Häggman, E-mail: hh@mm.unknown (email)
article id 4956, category Article
Paavo Yli-Vakkuri, Paavo Pelkonen. (1976). Rooting of Scots pine needle fascicles with different growth substances and media. Silva Fennica vol. 10 no. 4 article id 4956. https://doi.org/10.14214/sf.a14802
Keywords: Pinus sylvestris; Scots pine; vegetative propagation; rooting; needle fascicles; Jacobsen’s germination apparatus
Abstract | View details | Full text in PDF | Author Info

Needle fascicles of Scots pine (Pinus sylvestris L.) were rooted in a standard Jacobsen’s germination apparatus. The apparatus was found to be suitable for rooting at least on laboratory scale. The best rooting substrate was living Sphangnum, which remained sufficiently moist in the germination containers throughout the experiments. In a comparison of various growth substrate treatments, the best result was obtained with a combination of IAA (100 mg/l) and thiamine (5 mg/l). The rooting percentage using these growth substances with Sphagnum as the rooting medium was in the first experiment 30 and in the second 48.

The PDF includes a summary in Finnish.

  • Yli-Vakkuri, E-mail: py@mm.unknown (email)
  • Pelkonen, E-mail: pp@mm.unknown

Category : Research article

article id 1588, category Research article
Kouakou Laurent Kouakou, Jonas Patrick Dao, Kouadio Ignace Kouassi, Manehonon Martine Beugré , Mongomaké Koné, Jean-Pierre Baudoin, Irié Arsene Zoro Bi. (2016). Propagation of Garcinia kola (Heckel) by stem and root cuttings. Silva Fennica vol. 50 no. 4 article id 1588. https://doi.org/10.14214/sf.1588
Keywords: coppicing; vegetative propagation; bitter kola; non-mist polypropagators
Highlights: Roots and branches cuttings from mature forest trees are difficult to sprout; The highest mean numbers of roots and buds produced were obtained with softwood cuttings; The aqueous IBA treatment was more effective than the powder in promoting rooting and root development; Non-mist poly-propagator gave the best propagation results; Seedling stockplants cut above node 1 promoted most vigorous shoots production.
Abstract | Full text in HTML | Full text in PDF | Author Info

The availability of appropriate propagation techniques is a major constraint to the domestication of the forest trees widely used by rural communities; such as Garcinia kola (Heckel). This study tested the ability of root and stem cuttings to regenerate vegetatively when treated with indole-3-butyric acid (IBA), and set in non-mist poly-propagator within a shaded nursery. It found that G. kola is amenable to propagation by softwood stem cuttings. Attention was given to the effect of cutting age (softwood, semi hardwood) and IBA application with regard to the sprouting and rooting efficiency. Bud and leaf emergence time were also investigated, as was the coppicing ability of the stump. Results revealed that roots and branches from mature forest trees did not sprout under any culture conditions. The highest percentages of rooting (70–85%) were obtained with softwood cuttings set in non-mist poly-propagator, regardless of hormonal treatment. The mean numbers of buds (2.9 ± 0.4) and roots (2.6 ± 0.1) produced by softwood cuttings was significantly greater than those obtained in the Control. The best average emergence time of buds (25.1 days ± 9.3) and leaves (36.8days ± 8.4) was obtained with cuttings treated with IBA and set in non-mist poly-propagator against, 54.4 ± 12.5 and 72.6 ± 3.4 days respectively for the Control. In general, non-mist poly-propagator gave the best propagation results. When coppiced, the shoots emerging from stumps with one node were the most vigorous.

  • Kouakou, Université Nangui Abrogoua, UFR/SN, Laboratoire de Génomique Fonctionnelle et Amélioration génétique, 02 BP 801 Abidjan, Côte d’Ivoire; Université Nangui Abrogoua, UFR Sciences de la Nature, Laboratoire de Biologie et Amélioration des Productions Végétales, 02 BP 801 Abidjan 02, Côte d’Ivoire E-mail: kk_laurent@yahoo.fr (email)
  • Dao, Université Nangui Abrogoua, UFR/SN, Laboratoire de Génomique Fonctionnelle et Amélioration génétique, 02 BP 801 Abidjan, Côte d’Ivoire E-mail: daojonas@hotmail.fr
  • Kouassi, Université Nangui Abrogoua, UFR/SN, Laboratoire de Génomique Fonctionnelle et Amélioration génétique, 02 BP 801 Abidjan, Côte d’Ivoire E-mail: kouadioignace@yahoo.fr
  • Beugré , Université Nangui Abrogoua, UFR Sciences de la Nature, Laboratoire de Biologie et Amélioration des Productions Végétales, 02 BP 801 Abidjan 02, Côte d’Ivoire E-mail: beugremartine@yahoo.fr
  • Koné,  Université Nangui Abrogoua, UFR Sciences de la Nature, Laboratoire de Biologie et Amélioration des Productions Végétales, 02 BP 801 Abidjan 02, Côte d’Ivoire E-mail: babadaoudi@gmail.com
  • Baudoin, Université de Liège, Gembloux Agro-Bio Tech, Laboratoire Agroécologie tropicale et Horticulture, Passage des Déportés, 2 B 5030 Gembloux, Belgique E-mail: jean-pierre.baudoin@ulg.ac.be
  • Zoro Bi, Université Nangui Abrogoua, UFR/SN, Laboratoire de Génomique Fonctionnelle et Amélioration génétique, 02 BP 801 Abidjan, Côte d’Ivoire E-mail: banhiakalou@yahoo.fr
article id 452, category Research article
Catherine Ky-Dembele, Mulualem Tigabu, Jules Bayala, Patrice Savadogo, Issaka Joseph Boussim, Per Christer Odén. (2010). Clonal propagation of Detarium microcarpum from root cuttings. Silva Fennica vol. 44 no. 5 article id 452. https://doi.org/10.14214/sf.452
Keywords: Burkina Faso; sprouting efficiency; rootling; vegetative propagation
Abstract | View details | Full text in PDF | Author Info
Detarium microcarpum is a valuable tree species for fuelwood, timber, food and medicine in sub-Saharan Africa. However, its population is dwindling due to overexploitation, its seedlings’ low survival rate and slow growth. Vegetative propagation might enhance both survival and growth, but to date a successful clonal method does not exist for D. microcarpum. We conducted four experiments to examine the effects of propagation environment (high versus low humidity), cutting length and diameter, alignment of root segments (horizontal versus vertical) and distance from the root collar of donors on the regeneration ability of root segments collected from field-grown D. microcarpum trees in Burkina Faso. The size of root segments significantly affected their regeneration ability, while alignment had no effect. Sprouting was possible from 10 and 20-cm long segments of 15–60 mm diameter with 7–43% sprouting efficiency and multiple shoots while 5 cm long segments were unsuitable with 0–3% sprouting efficiency. Cuttings maintained at low humidity produced larger diameter sprouts than those in greenhouse. All cuttings showed strong polarity with most of the shoots developing at the proximal end. Rootlings from 20 cm root segments produced more new roots (0.62 ± 0.08 g) than those from 10 cm segments (0.34 ± 0.09 g), but they were similar for sprout and leaf growth. We conclude that lateral roots of field-grown mature trees can be used to produce rootlings in a nursery. Since this study is the first attempt to propagate D. microcarpum from root cuttings, further investigations are required to optimize the technique.
  • Ky-Dembele, Département Productions Forestières, Institut de l’Environnement et de Recherches Agricoles, 03 BP 7047 Ouagadougou 03, Burkina Faso & Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 101, SE-230 53 Alnarp, Sweden (catherine.dembele@ess.slu.se) E-mail: kydembele@hotmail.com (email)
  • Tigabu, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 101, SE-230 53, Alnarp, Sweden E-mail: mt@nn.se
  • Bayala, World Agroforestry Centre, West Africa and Centre Regional Office, Sahel Node, BP E5118 Bamako, Mali E-mail: jb@nn.ml
  • Savadogo, Département Productions Forestières, Institut de l’Environnement et de Recherches Agricoles, 03 BP 7047 Ouagadougou 03, Burkina Faso & Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 101, SE-230 53 Alnarp, Sweden E-mail: ps@nn.bf
  • Boussim, Université de Ouagadougou, Unité de Formation et Recherche en Sciences de la Vie et de la Terre, 03 BP 7021, Ouagadougou 03, Burkina Faso E-mail: ijb@nn.bf
  • Odén, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 101, SE-230 53, Alnarp, Sweden E-mail: pco@nn.se

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