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Articles containing the keyword 'differentiation'

Category : Article

article id 5269, category Article
Leena Ryynänen, Martti Ryynänen. (1986). Propagation of adult curly-birch succeeds with tissue culture. Silva Fennica vol. 20 no. 2 article id 5269. https://doi.org/10.14214/sf.a15448
Keywords: cell differentiation; Betula pendula var. carelica; tissue culture; clonal propagation; clonal variegation; plantlets
Abstract | View details | Full text in PDF | Author Info

The curly-grained trait of Betula pendula Roth is inheritable, but it is persumably not question of only one Mendelian gene since, for instance, there are a number of different types of curly-birch. The progeny obtained from controlled crossing between two curly-birch individuals do not all posses the curly-grained trait.

Plantlets were produced from adult curly-birch (Betula pendula var. carelica). Murashige and Skoog’s medium was used as the culture medium. Growth was initiated on a medium containing 1 mg/l BAP. Bud formation was induced using a medium containing 10 mg/l BAP and 0.2 mg/l NAA. Development of shoots was achieved on a medium containing ½ x Murashige and Skoog’s macrominerals and sucrose, 1/1 x Murashige and Skoog’s microminerals and vitamins, and 0.5 mg/l BAP and 0.5 mg/l IAA. The medium used for inducing root formation was the same as above, but without any growth regulators. The results indicate that adult deciduous trees can be best propagated through tissue culture when the least differentiated cells, i.e. the initial cells of the promeristem, are used as the startin material. The axillary buds provide easily available study material which can be prepared with little difficulty and are continuously renewed.

The PDF includes a summary in Finnish.

  • Ryynänen, E-mail: lr@mm.unknown (email)
  • Ryynänen, E-mail: mr@mm.unknown
article id 5117, category Article
Päiviö Riihinen. (1981). Forestry and the timber economy in economic development. Silva Fennica vol. 15 no. 2 article id 5117. https://doi.org/10.14214/sf.a15058
Keywords: forestry; forest industries; economic development; economic growth; differentiation
Abstract | View details | Full text in PDF | Author Info

The article deals with differences in economic growth in different countries and regions and with reasons for these differences. The central role in investments in economic growth and the mechanism of its differentiation are elucidated. The properties of forestry and the forest industries in equation or differentiating economic growth are considered. In the light of the theories of regional differentiation, the mere production of raw material in some region tends to increase differences in economic development if that raw material is processed in some other region – despite the fact that the level of income rises in both regions. It is therefore desirable that afforestation projects in the development countries are accompanied by the development of the forest industries.

The PDF includes a summary in Finnish.

  • Riihinen, E-mail: pr@mm.unknown (email)
article id 4926, category Article
Aarne Reunala. (1975). Metsänomistuksen muutokset ja aluepolitiikka. Silva Fennica vol. 9 no. 4 article id 4926. https://doi.org/10.14214/sf.a14768
English title: Forest ownership changes and regional development in Finland.
Original keywords: yksityismetsät; maatalousväestö; metsänomistus; aluekehitys; aluepolitiikka
English keywords: forest ownership; private forests; rural population; regional differentiation; regional policy
Abstract | View details | Full text in PDF | Author Info

The study sought to establish, whether a connection between forest ownership changes and regional differentiation process exists. Data were collected by interviewing fifty persons representing regional planning and forestry. In 1969–72 forest land area owned by the farmers decreased by some 600,000–700,000 hectares. The new owners were non-farmers (400,000–500,000 ha) and the State and forest industry companies (200,000 ha). These figures indicate a possibility for a reduction in the livelihood of rural developing regions in three ways: money incomes with their multiplier effects decrease, possibilities of the rationalization of farming decrease and the population confidence in the future diminishes.

The PDF includes a summary in English.

  • Reunala, E-mail: ar@mm.unknown (email)

Category : Research article

article id 1760, category Research article
Marek Fajstavr, Kyriaki Giagli, Hanuš Vavrčík, Vladimír Gryc, Josef Urban. (2017). The effect of stem girdling on xylem and phloem formation in Scots pine. Silva Fennica vol. 51 no. 4 article id 1760. https://doi.org/10.14214/sf.1760
Keywords: Pinus sylvestris; radial increment; cambial activity; cell differentiation; phloemogenesis; xylogenesis
Highlights: Stem girdling ceased the cambial activity, below the girdled area, immediately after the removal of the bark strip; Pinus sylvestris survived for up to two years after stem girdling; The girdled trees formed phloem cells above the girdled area but failed to form latewood cells in the next growing season.
Abstract | Full text in HTML | Full text in PDF | Author Info

Scots pine (Pinus sylvestris L.) is a resilient, wide spread species. This paper reports on the xylem and phloem cell formation process, before and after, the species was put under artificial stress by stem girdling. Microcore method was applied to a healthy control group and a standing group of girdled trees within an 80-year-old pine forest for two consecutive growing seasons (2013 and 2014). The stem girdling was applied in the middle of the first growing season (July 2013). Cambial activity timings (onset and cessation of cell division), cell formation intensity, cell differentiation, and the dynamics of the annual radial increment in the stem were analyzed. Cambial activity was inhibited and eventually ceased below the stem girdling immediately after the removal of the strip. Therefore, no latewood tracheids were formed. However, above the stem girdling and in the control trees, cell formation and tissue differentiation continued until the end of the growing season, with the girdled trees moving at a less intensive pace but for a longer period of time. During the following growing season (2014), the cambial zone was reactivated only above the stem girdling, not below, and eventually the girdled trees died. In 2014, the onset of the cambial activity was delayed and the division rate of the cells was slower in the girdled trees. Furthermore, the girdled trees formed less phloem cells than the control trees.

  • Fajstavr, Department of Wood Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 3, 613 00 Brno, Czech Republic E-mail: fajstavr.marek@seznam.cz (email)
  • Giagli, Department of Wood Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 3, 613 00 Brno, Czech Republic E-mail: kyriaki.giagli@mendelu.cz
  • Vavrčík, Department of Wood Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 3, 613 00 Brno, Czech Republic E-mail: vavrcik@mendelu.cz
  • Gryc, Department of Wood Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 3, 613 00 Brno, Czech Republic E-mail: gryc@mendelu.cz
  • Urban, Department of Forest Botany, Dendrology and Geobiocenology, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 3, 613 00 Brno, Czech Republic;  Siberian Federal University, Svobodnyj Prospect 79, Krasnoyarsk, 660041 Krasnoyarsk, Russia E-mail: josef.urban@email.cz
article id 1516, category Research article
Hamed Yousefzadeh, Abasalt Hosseinzadeh Colagar, Fatemeh Fallah. (2016). Genetic diversity of geographically isolated Iranian populations of Betula pendula Roth: implications for conservation. Silva Fennica vol. 50 no. 3 article id 1516. https://doi.org/10.14214/sf.1516
Keywords: genetic differentiation; habitat isolation; haplotype diversity; genetic drift; long-term isolation
Highlights: The Iranian populations of birches exhibited high levels of genetic diversity, population differentiation, and the presence of unique haplotypes; The high genetic differentiation amongst the populations may contribute to the local geographical structure and poor gene flow amongst individuals; The results can potentially be used to adopt appropriate strategies for the conservation and management of isolated tree populations.
Abstract | Full text in HTML | Full text in PDF | Author Info

The effects of long-term habitat fragmentations on genetic and population differentiation of Betula pendula Roth were investigated using chloroplast DNA (cpDNA) variations. Leaf samples were collected from four small remnant populations across the north of Iran. Three pairs of universal primers were used to amplify cpDNA, large single copy regions of trnC-trnD, trnK1-trnK2 and trnD-trnT. A total of 18 of the cpDNA haplotypes in the four populations were identified, however, no clear phylogeographic structuring of haplotypes could be detected. The total genetic diversity (HT) for all populations was high (0.932). Average intra-population genetic diversity was estimated as HS = 0.729 and average differentiation of populations GST = 0.218. Mantel tests of isolation by distance revealed a significant relationship between Wright’s inbreeding coefficient (Fst) and geographical distances for the four populations in Iran (r = 0.77, p < 0.05). The results of the hierarchical analysis of molecular variance (AMOVA) indicated that a 66% variation was partitioned within populations, whilst the variance amongst the four populations was only 34%. We suggest that significant genetic differentiation amongst populations can likely be attributed to reduced gene flow as a result of habitat fragmentation.

  • Yousefzadeh, Department of Forestry, Faculty of Natural Resources, Tarbiat Modares University, Nour, Mazandaran, Iran E-mail: h.yousefzadeh@modares.ac.ir (email)
  • Hosseinzadeh Colagar, Department of Molecular and Cell Biology; Faculty of Basic Sciences, University of Mazandaran, 47416-95447 Babolsar, Iran E-mail: ahcolagar@umz.ac.ir
  • Fallah, Department of Molecular and Cell Biology; Faculty of Basic Sciences, University of Mazandaran, 47416-95447 Babolsar, Iran E-mail: fatemehfalah69@yahoo.com
article id 1073, category Research article
Pedro Sánchez-Gómez, Juan F. Jiménez, Juan B. Vera, Francisco J. Sánchez-Saorín, Juan F. Martínez, Joseph Buhagiar. (2013). Genetic structure of Tetraclinis articulata, an endangered conifer of the western Mediterranean basin. Silva Fennica vol. 47 no. 5 article id 1073. https://doi.org/10.14214/sf.1073
Keywords: conservation; genetic differentiation; genetic diversity; ISSR markers
Highlights: The employment of ISSR molecular markers has shown moderate genetic diversity and high genetic differentiation in Tetraclinis articulata; Genetic structure of populations seems to be influenced by the anthropogenic use of this species since historical times, or alternatively, by the complex palaeogeographic history of the Mediterranean basin; Results could be used to propose management policies for conservation of populations.
Abstract | Full text in HTML | Full text in PDF | Author Info
Tetraclinis articulata (Vahl) Masters is a tree distributed throughout the western Mediterranean basin. It is included in the IUCN (International Union for Conservation of Nature) red list, and protected by law in several of the countries where it grows. In this study we examined the genetic diversity and genetic structure of 14 populations of T. articulata in its whole geographic range using ISSR (inter simple sequence repeat) markers. T. articulata showed moderate genetic diversity at intrapopulation level and high genetic differentiation. The distribution of genetic diversity among populations did not exhibit a linear pattern related to geographic distances, since all analyses (principal coordinate analysis, Unweighted pair group method with arithmetic mean dendrogram and Bayesian structure analysis) revealed that spanish population grouped with Malta and Tunisia populations. Although it is possible that T. articulata earlier was natural in Southeast Spain, results suggest that the current population has been reintroduced into the Iberian Peninsula in historical times, due to its utility in mining and building. In addition, results could be used to propose management guidelines for the conservation of T. articulata.
  • Sánchez-Gómez, Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain E-mail: psgomez@um.es
  • Jiménez, Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain E-mail: fjimenez@um.es (email)
  • Vera, Departamento de Biología Vegetal (Botánica), Universidad de Murcia, Campus de Espinardo s/n, E-30100 Murcia, Spain E-mail: jbveraperez@gmail.com
  • Sánchez-Saorín, Dirección General de Medio Ambiente, Consejería de Presidencia de la Región de Murcia, C/ Catedrático Eugenio Úbeda nº3, E-30071 Murcia, Spain E-mail: fjavier.sanchez3@carm.es
  • Martínez, Dirección General de Medio Ambiente, Consejería de Presidencia de la Región de Murcia, C/ Catedrático Eugenio Úbeda nº3, E-30071 Murcia, Spain E-mail: juanf.martinez@carm.es
  • Buhagiar, Argotti Herbarium and Gardens (UOM), Triq Vincenzo Bugeja Floriana VLT 16, Malta E-mail: joseph.buhagiar@um.edu.mt
article id 907, category Research article
Chunyu Zhang, Yazhou Zhao, Xiuhai Zhao, Klaus von Gadow. (2012). Species-habitat associations in a northern temperate forest in China. Silva Fennica vol. 46 no. 4 article id 907. https://doi.org/10.14214/sf.907
Keywords: species richness; spatial autocorrelation; dispersal limitations; indicator species; topographic differentiation
Abstract | View details | Full text in PDF | Author Info
This contribution identifies species-habitat associations in a temperate forest in north-eastern China, based on the assumption that habitats are spatially autocorrelated and species are spatially aggregated due to limited seed dispersal. The empirical observations were obtained in a large permanent experimental area covering 660 x 320 m. The experimental area was subdivided into four habitat types using multivariate regression tree (MRT) analysis. According to an indicator species analysis, 38 of the 47 studied species were found to be significant indicators of the MRT habitat types. The relationships between species richness and topographic variables were found to be scale-dependent, while the great majority of the species shows distinct habitat-dependence. There are 188 potential species-habitat associations, and 114 of these were significantly positive or negative based on habitat randomization. We identified 139 significant associations using a species randomization. A habitat is not a closed system it may be both, either a sink or a source. Therefore, additional to the randomization, the Poisson Cluster Model (PCM) was applied. PCM considers the spatial autocorrelation of species and habitats, and thus appears to be more realistic than the traditional randomization processes. It identified only 37 associations that were significant. In conclusion, the deviation from the random process, i.e. the high degree of species spatial mingling may be explained by persistent immigration across habitats.
  • Zhang, Key Laboratory for Forest Resources & Ecosystem Processes of Beijing, Beijing Forestry University, Beijing 100083, China E-mail: zcy_0520@163.com (email)
  • Zhao, Department of Landscape Architecture, School of Architecture, Tsinghua University, Beijing 100084, China E-mail: yz@nn.cn
  • Zhao, Key Laboratory for Forest Resources & Ecosystem Processes of Beijing, Beijing Forestry University, Beijing 100083, China E-mail: xz@nn.cn
  • von Gadow, Faculty of Forestry and Forest Ecology, Georg-August-University Göttingen, Büsgenweg 5, D-37077 Göttingen, Germany E-mail: KGadow@gwdg.de
article id 94, category Research article
Youhong Peng, Ke Chen. (2011). Phylogeographic pattern of Populus cathayana in the southeast of Qinghai-Tibetan Plateau of China revealed by cpSSR markers. Silva Fennica vol. 45 no. 4 article id 94. https://doi.org/10.14214/sf.94
Keywords: genetic differentiation; refugia; phylogeography
Abstract | View details | Full text in PDF | Author Info
The vegetation in the Qinghai-Tibetan Plateau is thought to be highly sensitive and more vulnerable to global climate change than that of other areas. The uplift of the plateau as well as the climatic oscillations during glacial periods had a profound impact on plant species distribution and genetic diversity there. In the present study, seven pairs of cpSSR (chloroplast Simple Sequence Repeat) primers were utilized to detect genetic varieties of Populus cathayana Rehd populations from their natural range in the southeastern areas of Qinghai-Tibetan Plateau. A total of 28 alleles and 12 different haplotypes were detected. The proportion of haplotype variation among populations (GST = 0.794, NST = 0.900) indicated high level of genetic differentiation among populations and a significant phylogeographic structure (NST > GST, P < 0.05). This appears to support the hypothesis that these populations were derived from multiple refugia areas during the Quaternary climatic oscillations. Based on the haplotype network and mismatch distribution analyses, we found no evidence of postglacial range recolonization and expansion by P. cathayana in this region. This might be mainly due to the complex topography of the southeastern part of the Qinghai-Tibetan Plateau. The lofty mountain ranges and deep valleys in this region might have prevented long-distance migrations of this species during the climatic amelioration.
  • Peng, Chinese Academy of Sciences, Chengdu Institute of Biology, Chengdu, China E-mail: pengyh@cib.ac.cn (email)
  • Chen, Chinese Academy of Sciences, Chengdu Institute of Biology, Chengdu, China E-mail: kc@nn.cn
article id 29, category Research article
Piotr Androsiuk, Roman Zielinski, Kornelia Polok. (2011). B-SAP markers derived from the bacterial KatG gene differentiate populations of Pinus sylvestris and provide new insights into their postglacial history. Silva Fennica vol. 45 no. 1 article id 29. https://doi.org/10.14214/sf.29
Keywords: Pinus sylvestris; KatG gene; molecular markers; population differentiation; migrations
Abstract | View details | Full text in PDF | Author Info
The aim of the studies was to evaluate the efficiency of the KatG gene based B-SAP markers as a tool to distinguish morphologically diversified and geographically distant Scots pine populations and to track the routes of migrations. The 19 populations growing in the IUFRO 1982 provenance experiment and representing the natural distribution of the species in Europe were scored using 103 B-SAP loci. Among them 26% loci were polymorphic. The level of polymorphism was associated with the location of primers on the KatG template. The diversity was low, He = 0.086, and deposited mostly among populations. Seven unique markers were found that identified populations and likely they were associated with morphology. The overall genetic identity was relatively low, I = 0.933 (D = 0.069). The block of six B-SAP markers discriminated populations into two groups in agreement with their geographic origin and thereby further described as the North and the South. The North group was uniform with genetic diversity, He = 0.026 and the overall genetic distance D = 0.022. Presumably, it migrated from refugia in the Alps via France, northern Germany and Denmark, to Scandinavia and Russia. The South group was heterogeneous with He = 0.063 and D = 0.047. This group migrated from the Carpathians via Slovakia to Germany and Poland. The Balkans and Asian refugia did not take part in recolonization of Europe. The block of six B-SAP/KatG markers can be recommended for tracking postglacial history of Scots pine.
  • Androsiuk, University of Warmia and Mazury in Olsztyn, Department of Genetics, Plac Lodzki 3, 10-967 Olsztyn, Poland E-mail: pa@nn.pl
  • Zielinski, University of Warmia and Mazury in Olsztyn, Department of Genetics, Plac Lodzki 3, 10-967 Olsztyn, Poland E-mail: rz@nn.pl
  • Polok, University of Warmia and Mazury in Olsztyn, Department of Genetics, Plac Lodzki 3, 10-967 Olsztyn, Poland E-mail: kpolok@moskit.uwm.edu.pl (email)
article id 205, category Research article
Pekka Vakkari, Mari Rusanen, Katri Kärkkäinen. (2009). High genetic differentiation in marginal populations of European white elm (Ulmus laevis). Silva Fennica vol. 43 no. 2 article id 205. https://doi.org/10.14214/sf.205
Keywords: marginal populations; genetic differentiation; genetic conservation; Ulmus
Abstract | View details | Full text in PDF | Author Info
Studies on the amount of genetic variation in marginal populations and differentiation between them are essential for assessment of best gene conservation strategies and sampling schemes. Thirteen marginal populations of Ulmus laevis in southern Finland and one in Estonia were investigated for genetic variation in 20 allozyme loci. Population differentiation among Finnish stands was high, Fst = 0.290, and mean genetic diversity low, He = 0.088. The differentiation follows the isolation-by-distance structure within the core of the distribution area (lake Vanajavesi). Fairly high frequency of recurrent genotypes was observed, but this did not have an influence on the genetic parameters. The observed genetic structure is consistent with the central-marginal hypothesis. In the light of the results, the Finnish gene conservation strategy for U. laevis seems to be on a sound basis.
  • Vakkari, Finnish Forest Research Institute, Vantaa Research Unit, P.O. Box 18, FI-01301 Vantaa, Finland E-mail: pekka.vakkari@metla.fi (email)
  • Rusanen, Finnish Forest Research Institute, Vantaa Research Unit, P.O. Box 18, FI-01301 Vantaa, Finland E-mail: mr@nn.fi
  • Kärkkäinen, Finnish Forest Research Institute, Vantaa Research Unit, P.O. Box 18, FI-01301 Vantaa, Finland E-mail: kk@nn.fi
article id 314, category Research article
Yuhua Wang, Helena Korpelainen, Chunyang Li. (2006). Microsatellite polymorphism in the edaphic spruce, Picea asperata, originating from the mountains of China. Silva Fennica vol. 40 no. 4 article id 314. https://doi.org/10.14214/sf.314
Keywords: genetic diversity; microsatellite polymorphism; micro-geographic differentiation; natural selection; mutation
Abstract | View details | Full text in PDF | Author Info
Microsatellite variation of Picea asperata Mast. originating from the mountains of China was investigated by analyzing variation at seven SSR loci in 250 individuals representing ten populations. A fair degree of genetic diversity and considerable population subdivision occurred with the mean gene diversity (H) of 0.707, and genetic distances among populations varying between 0.121 and 0.224 (FST) and between 0.100 and 0.537 (RST). However, inter-population genetic distances showed no correlation with geographic distances between the population sites. This ruled out a simple isolation by distance model and suggested that migration does not have a great impact. In fact, the amount of gene flow, detected using private alleles, was very low, equaling only 0.753. Allele permutation tests revealed that stepwise-like mutations, coupled with genetic drift, could contribute to population differentiation. Moreover, significant genetic differences between populations were detected at most loci. The results indicate that natural selection, presumably through environmental stress, may be one of the main factors causing micro-geographical differentiation in the genetic structure of P. asperata. Based on SSR genotypes, 70% of the 250 individuals were correctly classified into their sites of origin. This suggests that microsatellites (SSRs) are effective in distinguishing genotypes of P. asperata originating from diverse eco-geographical sites in China.
  • Wang, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, China E-mail: yw@nn.cn
  • Korpelainen, Department of Applied Biology, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: hk@nn.fi
  • Li, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, China E-mail: licy@cib.ac.cn (email)
article id 476, category Research article
Ignazio Monteleone, Diana Ferrazzini, Piero Belletti. (2006). Effectiveness of neutral RAPD markers to detect genetic divergence between the subspecies uncinata and mugo of Pinus mugo Turra. Silva Fennica vol. 40 no. 3 article id 476. https://doi.org/10.14214/sf.476
Keywords: molecular markers; population differentiation; genetic diversity; Pinus mugo; mountain pine; dwarf mountain pine
Abstract | View details | Full text in PDF | Author Info
Fifteen populations of Pinus mugo subsp. mugo (shrub) and Pinus mugo subsp. uncinata (erect), located in the Alps, were investigated through genetic variation scored at 64 polymorphic RAPD loci. In addition, morphological traits of the female cones were analysed. According to AMOVA most of the genetic variation was found within populations (83.39%), and only 1.25% of it between subspecies. Populations differed in terms of their internal genetic variation, with Nei’s gene diversity ranging from 0.227 to 0.397. Morphological data showed differences between subspecies, although none of the populations showed full accordance with expectations. Significant correlation was found between matrices for geographical and morphological distances, while genetic distances were not correlated with any other aspect. The efficacy of morphological and RAPD markers in discriminating between subspecies, and the contribution of the results in relation to the preservation of biodiversity, are discussed.
  • Monteleone, DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy E-mail: im@nn.it
  • Ferrazzini, DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy E-mail: df@nn.it
  • Belletti, DI.VA.P.R.A., Plant Genetics and Breeding, University of Turin, via Leonardo da Vinci 44, I-10095 Grugliasco, Italy E-mail: piero.belletti@unito.it (email)
article id 326, category Research article
Xuejiang Zhang, Helena Korpelainen, Chunyang Li. (2006). Microsatellite variation of Quercus aquifolioides populations at varying altitudes in the Wolong Natural Reserve of China. Silva Fennica vol. 40 no. 3 article id 326. https://doi.org/10.14214/sf.326
Keywords: genetic differentiation; genetic variation; altitudinal gradients; microsatellites; Quercus aquifolioides
Abstract | View details | Full text in PDF | Author Info
Genetic variation and differentiation were investigated among five natural populations of Quercus aquifolioides occurring along an altitudinal gradient that varied from 2000 to 3600 m above sea level in the Wolong Natural Reserve of China, by analyzing variation at six microsatellite loci. The results showed that the populations were characterized by relatively high intra-population variation with the average number of alleles equaling 11.33 per locus and the average expected heterozygosity (HE) being 0.779. The amount of genetic variation varied only little among populations, which suggests that the influence of altitude factors on microsatellite variation is limited. However, there is a significantly positive correlation between altitude and the number of low-frequency alleles (R2 = 0.97, P < 0.01), which indicates that Q. aquifolioides from high altitudes has more unique variation, possibly enabling adaptation to severe conditions. F statistics showed the presence of a slight deficiency of heterozygosity (FIS = 0.136) and a low level of differentiation among populations (FST = 0.066). The result of the cluster analysis demonstrated that the grouping of populations does not correspond to the altitude of the populations. Based on the available data, it is likely that the selective forces related to altitude are not strong enough to significantly differentiate the populations of Q. aquifolioides in terms of microsatellite variation.
  • Zhang, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, P. R. China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, P. R. China E-mail: xz@nn.cn (email)
  • Korpelainen, Department of Applied Biology, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: hk@nn.fi
  • Li, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, P. R. China E-mail: licy@cib.ac.cn
article id 663, category Research article
Lennart Moberg. (1999). Variation in knot size of Pinus sylvestris in two initial spacing trials. Silva Fennica vol. 33 no. 2 article id 663. https://doi.org/10.14214/sf.663
Keywords: site quality; silviculture; stand density; azimuth; initial spacing; knot size; within-stand differentiation
Abstract | View details | Full text in PDF | Author Info
The objective of this study was to investigate the variation in internal knot size of Scots pine (Pinus sylvestris L.) stems sampled from mature permanent plots, and for which the silvicultural history was known. It was based on a sample of mature trees removed from two different spacing trials representing a moderate and high site index. Knot size was measured with non-destructive methods using a CT-scanner and digital image analysis. Initial spacing varied between 0.75 and 3 m on the high site-index trial and between 1.5 and 2.5 m on the moderate site-index trial. Wider initial spacing on the high site index resulted in larger knots near the base of the stem. However, due to successive thinnings which gradually equalised stand density among plots, the difference between most plots was less further up in the stems. The effect of silvicultural regime was much more limited on the lower site index. Within-stand differentiation resulted in a variation of tree diameter (DBH); larger trees had significantly larger knots. Furthermore, knots were larger towards south than towards north in both trials. These results illustrated that, by using non-destructive measurements on trees sampled from permanent research plots, it was possible to simultaneously study the variation of internal knot size at stand (such as site and silviculture effects), within-stand (such as relative tree size) and within-tree levels (such as height and azimuth). However, lack of replication prevented valid statistical inference as to stand-level effects.
  • Moberg, Swedish University of Agricultural Sciences, Department of Forest Management and Products, P.O. Box 7060, S-750 07 Uppsala, Sweden E-mail: lennart.moberg@sh.slu.se (email)

Category : Research note

article id 1415, category Research note
Zorica Šarac, Tanja Dodoš, Nemanja Rajčević, Srdjan Bojović, Petar Marin, Jelena Aleksić. (2015). Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data. Silva Fennica vol. 49 no. 5 article id 1415. https://doi.org/10.14214/sf.1415
Keywords: genetic differentiation; genetic diversity; the Balkans; European black pine; chloroplast microsatellites; mitochondrial nad7 intron 1; haplotype network
Highlights: Seven populations of Pinus nigra from the central Balkans, representing four infraspecific taxa, were analyzed with chloroplast microsatellites and a mitochondrial locus; Molecular data failed to support infraspecific circumscriptions; Levels of genetic diversities/differentiation at both genomes were in the range of those reported in western Mediterranean populations of P. nigra; Iberian/African and Balkans’ populations share one mtDNA polymorphism and differ in three mutations.
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Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Đorđević; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is – exceptionally high levels of genetic diversity (HT = 0.949) and low genetic differentiation (GST = 0.024) at the cpDNA level, and moderate levels of genetic diversity (HT = 0.357) and genetic differentiation (GST = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans’ and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.

  • Šarac, University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology, Višegradska 33, 18000 Niš, Serbia E-mail: saraczorica@gmail.com (email)
  • Dodoš, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: tanjadodos@bio.bg.ac.rs
  • Rajčević, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: nemanja@bio.bg.ac.rs
  • Bojović, University of Belgrade, Institute for Biological Research “Siniša Stanković”, Boulevard Despota Stefana 142, 11060 Belgrade, Serbia E-mail: bojovic@ibiss.bg.ac.rs
  • Marin, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: pdmarin@bio.bg.ac.rs
  • Aleksić, University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11000 Belgrade, Serbia E-mail: aleksic_jelena@yahoo.com.au

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